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适于基因组测序的高纯度的大豆线粒体基因组DNA提取方法 Title:AHigh-PuritySoybeanMitochondrialGenomeDNAExtractionMethodforGenomicSequencing Abstract: Genomicsequencingplaysacrucialroleinunderstandingthegeneticbasisoftraitsandexploringvariationswithinanorganism'sgenome.Soybeanhasgainedsignificantattentionduetoitsagriculturalimportanceandpotentialapplicationsinindustry.However,extractinghigh-puritymitochondrialgenomeDNAfromsoybeanremainsachallenge.Inthisstudy,wepresentanovelandefficientDNAextractionmethodspecificallytailoredforsoybeanmitochondrialgenomes.Theproposedmethodcombinestraditionalandinnovativetechniques,resultinginhigh-quality,high-purityDNAsamplessuitableforgenomicsequencingapplications. 1.Introduction Advancementsingenomicsequencingtechnologiesofferunprecedentedopportunitiesforresearcherstostudythegeneticbasisoftraitsinvariousorganisms,includingcrops.Soybean(Glycinemax)isutilizedgloballyasavaluablecropduetoitshighproteinandoilcontent,makingitessentialforbothfoodandindustrialapplications.Understandingthemitochondrialgenomeofsoybeanisofparticularinterestasitplaysacrucialroleintheplant'senergymetabolismandotherbiologicalprocesses.However,obtaininghigh-puritymitochondrialDNAfromsoybeanforgenomicsequencingremainsasignificantchallengethatneedstobeaddressed. 2.CurrentChallengesinSoybeanMitochondrialGenomeDNAExtraction SoybeanmitochondrialDNAextractionisproblematicduetoitscomplexityandthepresenceofcontaminants,suchaspolysaccharides,polyphenols,andproteins,whichcaninterferewithdownstreamapplications.Currentextractionmethodsoftenyieldlow-qualityDNA,limitingtheaccuracyandreliabilityofsubsequentgenomicsequencinganalyses.Therefore,itisimperativetodeveloparobustandefficientprotocoltoextracthigh-puritymitochondrialDNAfromsoybeanforaccurategenomicsequencing. 3.Methodology 3.1SampleCollectionandPreparation Freshsoybeanleaftissuefromhealthyandmatureplantsshouldbecollectedandstoredat-80°Cuntiluse.Priortoextraction,theleaftissueshouldbegroundintofinepowderusingliquidnitrogenandasterilemortarandpestle. 3