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酶转化在淫羊藿苷提取中的应用.docx

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酶转化在淫羊藿苷提取中的应用 Title:ApplicationofEnzymaticConversioninEpimediumGlycosideExtraction Abstract: Enzymaticconversionhasgainedincreasingattentioninvariousindustrialprocesses,includingtheextractionofnaturalcompounds.Inthispaper,weexploretheapplicationofenzymaticconversionintheextractionofEpimediumglycosides,specificallyfocusingonthetransformationoficariinintoitsmajoractivemetabolite,icaritin.Thepaperdiscussestheadvantagesofenzymaticconversionovertraditionalextractionmethodsandhighlightsthevariousenzymesandreactionconditionsutilizedinthisprocess.Additionally,thepaperpresentspotentialchallengesfacedintheenzymaticconversionofEpimediumglycosidesandprovidesfutureperspectivesforfurtherresearchanddevelopment.Overall,enzymaticconversionoffersapromisingalternativefortheextractionofEpimediumglycosides,contributingtothedevelopmentofefficientandsustainableextractionprocesses. 1.Introduction: Epimedium,commonlyknownasHornyGoatWeed,isagenusoffloweringplantsbelongingtotheBerberidaceaefamily.Itiswidelyrecognizedforitsvarioushealthbenefits,particularlyitspotentialasanaphrodisiacandforthetreatmentofosteoporosis.TheactiveconstituentsresponsiblefortheseeffectsaretheEpimediumglycosides,withthemostabundantandbioactivecompoundbeingicariin.Whileicariinhasdemonstratedtherapeuticproperties,italsoundergoesenzymaticconversioninthehumanbodyintoicaritin,itsmorepotentmetabolite.Thus,thetransformationoficariinintoicaritinhasbecomeafocusofresearchforthedevelopmentofimprovedEpimediumglycosideextracts. 2.EnzymaticConversionProcess: Enzymaticconversionutilizesspecificenzymestocatalyzethetargetedtransformationoficariinintoicaritin.Oneofthemostcommonlyemployedenzymesinthisprocessisβ-glucosidase.Byhydrolyzingtheglucosemoietyattachedtoicariin,β-glucosidaseconvertsicariinintoicaritin.Thisenzymaticconversionoffersseveraladvantagesovertraditionalextractionmethods,includinghighselectivity,mildreactionconditions,andreducedenvironmentalimpact.Enzymaticconversioncanalsobeoptimizedtoenhancetheformationoficaritinandmin