史巧燕 竹荪不同部位抑菌效果的研究.docx

摘要本文主要针对长裙竹荪的菌裙、菌柄、菌盖、菌托等各不同部位的乙酸乙酯提取物进行了抑菌方面的研究。以大肠杆菌、金黄色葡萄球菌和沙门氏菌为实验菌株通过平板打孔法以抑菌圈直径为测量指标对抑菌效果进行评价；采用连续倍比浓度稀释法来确定竹荪不同部位提取物的最低抑菌浓度（MIC值）比较四个部位的抑菌效果。结果如下：（1）长裙竹荪菌柄提取物的抑菌效果是四个部位中最好的对大肠杆菌、金黄色葡萄球菌和沙门氏菌的MIC值都是25mg/ml且对金黄色葡萄球菌的抑制效果最好；（2）长裙竹荪菌裙的提取物对大肠杆菌的抑菌效果最好MIC值是50mg/ml；（3）长裙竹荪菌盖的提取物对沙门氏菌的抑菌效果最好MIC值是25mg/ml；（4）长裙竹荪菌托的提取物对金黄色葡萄球菌和沙门氏菌抑菌效果比较好MIC值都是50mg/ml。关键词：长裙竹荪；乙酸乙酯提取物；抑菌圈直径；最低抑菌浓度AbstractInthispapertheantibacterialactivitiesofethylacetateextractsfromdifferentpartsofDictyophoraindusiatasuchasfungusskirtfungusstemfunguscoverandv-olvawerestudied.TakingEscherichiacolistaphylococcusaureusandsalmonellaasexperimentalstrainsthebacteriostaticeffectwasevaluatedbytheplatepunchingm-ethodandthediameterofbacteriostaticcircleasmeasurementindex.Theminimuminhibitoryconcentration(MIC)ofextractsfromdifferentpartsofDictyophoraindusi-atawasdeterminedbycontinuousmultipledilutionmethodandtheantibacterialeff-ectsofthefourpartswerecompared.Theresultsareasfollows:(1)TheantibacterialeffectofDictyophoraindusiatastipeextractisthebestamongthefourpartsMICva-luesforEscherichiacoliStaphylococcusaureusandSalmonellaareall25mg/mlandtheinhibitoryeffectonStaphylococcusaureusisthebest.(2)TheextractofDictyop-horaindusiatamaximmaximmaximhasthebestantibacterialeffectonEscherichiacoliMICvalueis50mg/ml；;(3)TheextractofDictyophoraindusiatacaphastheb-estantibacterialeffectonSalmonellaMICvalueis25mg/mL;(4)TheextractofDc-tyophoraindusiatavolvahasgoodantibacterialeffectonStaphylococcusaureusandSalmonellaMICvaluesare50mg/ml.Keywords:Dictyophoraindusiata;Ethylacetateextract;Diameterofbacteriostaticci-rcle;Minimuminhibitoryconcentratio－２０－目录1引言１1.1竹荪的简介１1.2竹荪抑菌效果的研究现状１1.3研究的目的及意义２1.4研究内容２2材料与方法３2.1实验材料及仪器３2.1.1材料３2.1.2试剂３2.1.3仪器４2.2实验方法４2.2.1长裙竹荪乙酸乙酯提取物的制备４2.2.2抑菌试验准备４2.2.3抑菌效果的测定５2.2.4数据处理６3结果与分析６3.1竹荪不