酚氨咖敏颗粒含量测定方法的改进.docx

酚氨咖敏颗粒含量测定方法的改进[摘要]目的：建立HPLC法同时测定氨酚咖敏颗粒中对乙酰氨基酚、咖啡因、氨基比林的含量。方法：采用HPLC法色谱柱：KromasilC18(4.6mm×250mm5μm)；流动相：1%醋酸溶液（用三乙胺调pH为3.5）-甲醇-乙腈（65∶10∶25）；流速：1.0ml/min；检测波长：273nm；柱温：30℃。结果：对乙酰氨基酚检测浓度的线性范围为12.3～123.2μg/ml（r=0.9996）平均回收率为98.65%RSD=0.31%（n=3）；咖啡因检测浓度的线性范围为2.44～24.42μg/ml（r=0.9998）平均回收率为99.15%RSD=0.15%（n=3）；氨基比林检测浓度的线性范围为8.23～82.34μg/ml（r=0.9999）平均回收率为98.40%RSD=0.39%（n=3）。结论：本法灵敏可靠简单可行可用于氨酚咖敏颗粒中对乙酰氨基酚、咖啡因、氨基比林的含量测定。[关键词]氨酚咖敏颗粒；对乙酰氨基酚；咖啡因；氨基比林；HPLC[中图分类号]R927.2[文献标识码]A[文章编号]1673-7210（2010）11（b）-044-03SimultaneousdeterminationofParacetamolCaffeineAminopyrineinParacetamolaminophenazonephenacetincaffeineandchlorphenaminemaleateGranulesJIAOFuzhe1GAOJianling2(1.NanyangHealthSchoolforMedicalWorkersHe'nanProvinceNanyang473000China;2.NanyangMedicalCollegeHe'nanProvinceNanyang473000China)[Abstract]Objective:ToestablishamethodfordeterminationofParacetamolCaffeineAminopyrineinParacetamolaminophenazonephenacetincaffeineandchlorphenaminemaleateGranulesbyHPLC.Methods:TheKromasilC18column(4.6mm×250mm5μm)wasusedamixtureof1%-Aceticacid(adjustpHto3.5bytriethylamine)-methanol-acetonitrile(65∶10∶25）asthemobilephase.Thedetectionwavelengthwasat273nmtheflowratewas1.0ml/min.Thetemperatureofthecolumnwasat30℃.Results:ThelinearofrangeParacetamolwasat12.3-123.2μg/ml(r=0.9996)theaveragerecoverywas98.65%withaRSDof0.31%(n=3);thelinearrangeofCaffeinewas2.44-24.42μg/ml(r=0.9998)theaveragerecoverywas99.15%withaRSDof0.15%(n=3);thelinearrangeofAminopyrinewas8.23-82.34μg/ml(r=0.9999)theaveragerecoverywas98.40%withaRSDof0.39%(n=3).Conclusion:Themethodissimplereliableandsensitive.ThismethodcanbeusedforthedeterminationofParacetamolCaffeineAminopyrineinParacetamolaminophenazonephenacetincaffeineandchlorphenaminemaleateGranules.[Keywords]Paraceta