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复方柴黄软胶囊提取物测定分析研究 Title:DeterminationandAnalysisoftheExtractsofCompoundChaihuangSoftCapsules Abstract: CompoundChaihuangSoftCapsulesaretraditionalChinesemedicineswidelyusedforthetreatmentofvariousdiseases.ThisstudyaimstodetermineandanalyzethechemicalcompositionandphytoconstituentspresentintheextractsofCompoundChaihuangSoftCapsules.Variousanalyticaltechniquessuchaschromatographyandspectroscopywereemployedfortheidentificationandquantificationofactivecompounds.TheresultsprovidevaluableinsightsintothequalitycontrolandstandardizationofCompoundChaihuangSoftCapsules. Introduction: TraditionalChinesemedicineplaysasignificantroleinhealthcare,offeringawiderangeoftherapeutictreatments.CompoundChaihuangSoftCapsules,atraditionalherbalmedicineformulationcommonlyusedinChina,havebeenshowntopossessnumerouspharmacologicaleffects,includinganti-inflammatory,immunomodulatory,andhepatoprotectiveactivities.However,thechemicalcompositionandidentificationofbioactivecompoundsinCompoundChaihuangSoftCapsuleshavenotbeenextensivelyinvestigated.Therefore,thisstudyaimedtofillthisresearchgapbyconductingadetailedanalysisoftheextractsofCompoundChaihuangSoftCapsules. Methods: TheextractsofCompoundChaihuangSoftCapsuleswerepreparedusingappropriateextractiontechniques.Thequalitativeanalysiswasperformedusingthin-layerchromatography(TLC)andhigh-performanceliquidchromatography(HPLC).Theidentificationandquantificationofselectedactivecompoundsweredoneusingtechniquessuchasgaschromatography-massspectrometry(GC-MS),liquidchromatography-massspectrometry(LC-MS),andnuclearmagneticresonance(NMR)spectroscopy.Standardreferencecompoundswereusedfortheidentificationandquantificationoftheactivecompounds. ResultsandDiscussion: TheanalysisoftheextractsofCompoundChaihuangSoftCapsulesrevealedthepresenceofvariousphytoconstituents.Someoftheidentifiedcompoundsincludedflavonoids,terpenoids,alkaloids,andphenoliccompounds.TheidentificationandquantificationofthesecompoundswereessentialforassessingthequalityandpotencyofCompoundChaihua