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啤酒酵母复合酶法生产酵母抽提物工艺的研究.docx

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啤酒酵母复合酶法生产酵母抽提物工艺的研究 Title:StudyontheProductionProcessofYeastExtractfromBeerYeastbytheCombinationofBeerYeastComplexEnzymeMethod Abstract: Yeastextractisavaluableingredientwidelyusedinthefood,pharmaceutical,andcosmeticindustriesduetoitshighnutritionalandfunctionalproperties.Inthisstudy,weinvestigatedtheproductionprocessofyeastextractfrombeeryeastusingthecombinationofbeeryeastcomplexenzymemethod.Theenzymatichydrolysisprocesswasoptimizedbyexaminingtheeffectsofdifferentparameters,includingenzymedosage,hydrolysistime,pH,andtemperature.Theobtainedyeastextractwasthencharacterizedforitsnutritionalcompositionandfunctionalproperties.Theresultsshowedthatthecombinationofbeeryeastcomplexenzymemethodwasaneffectiveapproachfortheproductionofyeastextractwithenhancednutritionalandfunctionalproperties. 1.Introduction: Yeastextractisacomplexmixtureofproteins,peptides,carbohydrates,vitamins,minerals,andotherbioactivecomponentsderivedfromyeastcells.Ithasbeenwidelyutilizedasanutrientsourceandflavorenhancerinvariousapplications,includingfood,pharmaceutical,andcosmeticindustries.Duetoitsrichnutritionalprofileandfunctionalproperties,thedemandforyeastextracthasbeengrowingrapidlyinrecentyears.However,traditionalmethodsofyeastextractproduction,suchasautolysisandautolysiswithenzymatichydrolysis,oftensufferfromlowextractionefficiencyandlimitedyield.Therefore,exploringnewmethodsforefficientyeastextractproductionisofgreatimportance. 2.MaterialsandMethods: 2.1YeastStrainandCulture: Beeryeaststrain(Saccharomycescerevisiae)wasusedasthemicrobialsourceforyeastextractproduction.Theyeaststrainwasculturedinasuitablemediumunderoptimizedfermentationconditions. 2.2EnzymaticHydrolysisProcess: Thebeeryeastcellswereharvested,washed,andconcentrated.Theconcentratedyeastcellswerethentreatedwithacombinationofbeeryeastcomplexenzymes,includingβ-glucanase,protease,andglucanase.Thehydrolysisprocesswasoptimizedbyvaryingtheenzymedosage,hydrolysistime,pH,andtemperature. 2.3CharacterizationofYeastExtract: Theobtainedyeastex