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响应面法优化莜麦蛋白的酶解工艺及酶解物抗氧化活性的研究.docx

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响应面法优化莜麦蛋白的酶解工艺及酶解物抗氧化活性的研究 Title:OptimizationofEnzymaticHydrolysisofOatProteinUsingResponseSurfaceMethodologyandInvestigationofAntioxidantactivityofHydrolysates Abstract: Oatproteinisanexcellentsourceofessentialaminoacidsandbioactivepeptides,whichareknownfortheirpotentialantioxidantproperties.Enzymatichydrolysisisaneffectivemethodtoobtainproteinhydrolysateswithimprovedfunctionalproperties.Inthisstudy,responsesurfacemethodology(RSM)wasemployedtooptimizetheenzymatichydrolysisprocessofoatproteinandtoinvestigatetheantioxidantactivityofthehydrolysates.Theeffectsofthreekeyfactors,includingenzyme-to-substrateratio,hydrolysistime,andpH,wereevaluatedusingacentralcompositedesign.Theresultsrevealedsignificantinteractionsandquadraticeffectsofthesefactorsontheextentofhydrolysisandantioxidantactivityofoatproteinhydrolysates.Theoptimizedconditionsweredetermined,andtheresultinghydrolysateswerecharacterizedfortheirantioxidantactivityusingvariousinvitroassays. 1.Introduction: Oatproteinisgainingpopularityduetoitshighnutritionalvalueandpotentialhealthbenefits.Itcontainsauniquebalanceofessentialaminoacids,dietaryfibers,andbioactivepeptides.Bioactivepeptidesderivedfromoatproteinhaveshownvariousbiologicalactivities,includingantioxidant,antihypertensive,andimmunomodulatoryproperties.Enzymatichydrolysishasbeenwidelyusedtogenerateproteinhydrolysateswithimprovedfunctionality.Theoptimizationofenzymatichydrolysisparametersiscrucialtomaximizetheyieldofbioactivepeptidesandenhancetheirantioxidantactivity. 2.MaterialsandMethods: 2.1Materials: -Oatproteinisolate -Alcalase®2.4Lenzyme -Substratebuffer -Antioxidantassaysreagents(DPPH,FRAP,ABTS) 2.2EnzymaticHydrolysisOptimization: Acentralcompositedesign(CCD)wasusedtoinvestigatetheeffectsofenzyme-to-substrateratio(X1),hydrolysistime(X2),andpH(X3)ontheresponsevariables,includingdegreeofhydrolysis(DH)andantioxidantactivity.Thedesignmatrixwasgenerated,andthehydrolysisexperimentswereperformedaccordingly. 2.3DHDetermination: TheDHoftheoatproteinhydrolysate