HPLC法测量山豆根和开喉剑喷雾剂中氧化苦 参碱含量.pdf

HPLC法测量山豆根和开喉剑喷雾剂中氧化苦参碱含量--HPLC法测量山豆根和开喉剑喷雾剂中氧化苦参碱含量HPLC法测量山豆根和开喉剑喷雾剂中氧化苦参碱含量（贵州师范大学化学与材料学院贵州贵阳550001）摘要：以超声提取法提取山豆根与开喉剑中的氧化苦参碱为研究对象并采用高效液相色谱测定其出锋面积通过标准曲线方程计算出山豆根和开喉剑中氧化苦参碱的含量。甲醇-水（0.025％磷酸）（5:95）为流动相；检测波长：220nm；流速：1.0ml/min；进样量：10μl；柱温：40℃。结果表明：氧化苦参碱的HPLC进样量在10μg/ml-0.1μg/ml范围内与峰面积积分值呈良好线性关系（r=0.9995）标准偏差RSD=0.011平均回收率为76.43%。本方法简便易行、精确度高、重复性好。适用于检测山豆根和中成药中氧化苦参碱的含量。关键词：高效液相色谱法；山豆根；开喉剑；苦参碱DeterminationofOxymatrineinVietnameseSophoraRootandopenthelarynxswordwithhighperformanceliquidchromatographyLinghuChangfa(ChemistryandmaterialsscienceGuizhouNormalUniversity550001GuizhouGuiyang)Abstract:ExtractedbyultrasonicextractiontonkinensiswithopenthroatSwordoxymatrineforthestudyandusinghigh-performanceliquidchromatographicdeterminationofitsfrontareacalculatedbythestandardcurveequationtonkinensisandopenthroatswordinoxidationflavescensthecontentofabase.Methanol-water(0.025%phosphoricacid)(5:95)asmobilephase;detectionwavelength:220nm;flowrate:1.0ml/min;injectionvolume:10μl;columntemperature:40°C.:OxymatrineTheHPLCinjectionvolumein10μg/ml-0.1μg/mlwithintheintegralvalueofthepeakareashowedagoodlinearrelationship(r=0.9995)thestandarddeviation(RSD)=0.011theaveragerecoverywas76.43%.Thismethodissimplehighaccuracygoodrepeatability.ApplytodetectionoxymatrinetonkinensisandproprietaryChinesemedicinesinthecontent.Keywords:HPLC；VietnameseSophoraRoot；openthelarynxswordoxymatrine引言：山豆根（VietnameseSophoraRoot）为双子叶植物纲豆科植物越南槐的干燥根及根茎。植物形态为灌木高