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小胶质细胞在七氟烷预处理大鼠脊髓缺血再灌注损伤中的作用的研究及机制.docx

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小胶质细胞在七氟烷预处理大鼠脊髓缺血再灌注损伤中的作用的研究及机制 Introduction Spinalcordischemia-reperfusioninjury(SCII)isaseriouscomplicationthatcanoccurduringaorticsurgery,spinalcordinjuries,andothermedicalconditions.Theinjuryresultsinneuronalandglialcelldeath,impairedbloodflow,andreducedmotorandsensoryfunction.SeveralstudieshaveattemptedtoidentifythemechanismsofSCIIinjuryanddeveloptherapeuticstrategiestopreventorreduceitseffects.Onesuchmechanismistheactivationofmicrogliaandastrocytes,whicharetypesofglialcellsinvolvedintheimmuneresponseandtissuerepairafterinjury. Smallglialcells,ormicrogliacells,areimmunecellsthatplayavitalroleinthebrainandspinalcord.Undernormalconditions,theyareinarestingstateandactasscavengers,removingdebrisandmaintainingtheappropriatebalanceofchemicalsinthebrain.However,whenthespinalcordisinjured,microgliacellsareactivatedandbegintoreleaseinflammatorycytokinesandreactiveoxygenspecies(ROS)thatcanleadtocelldamageanddeath. Previousstudieshaveindicatedthatsevoflurane,acommonlyusedanesthetic,hasneuroprotectiveeffectsthatcanreducetheseverityofSCIIinjury.However,themechanismsbywhichsevofluraneexertsitsprotectiveeffectsarenotfullyunderstood.Recentstudiessuggestthatsevofluranecanmodulatetheactivationofmicrogliacellsbyinfluencingtheexpressionofinflammatorycytokinesandoxidativestressfactors. Inthisstudy,weaimedtoinvestigatetheroleofmicrogliacellsinSCIIinjuryandthepotentialmechanismsbywhichsevofluranemayprotectspinalcordtissuethroughtheregulationofmicrogliacellactivity. MaterialsandMethods Animalmodel ThirtyadultmaleSprague-Dawleyrats(weighing250-300g)wereobtainedfromtheAnimalResearchCenteratourinstitution.Theratswerehousedinindividualcagesandgivenunrestrictedaccesstofoodandwater.AllanimalexperimentswereapprovedbytheEthicsCommitteeofourinstitutionandconductedaccordingtotheGuidelinesfortheCareandUseofLaboratoryAnimals. Afteranesthesiawith3-5%isoflurane,theratswereplacedinapronepositionandunderwentlaminectomyatthelevelofT11-12.Theratswererandomlydividedintothreegroups:thecontrolgroup(n=10