纳米抗体靶向的纳米微泡的制备及其肾癌靶向性验证.docx

纳米抗体靶向的纳米微泡的制备及其肾癌靶向性验证
Title:PreparationandRenalCancerTargetingValidationofNanovesiclesTargetedbyNanobody
Abstract:
Renalcancerisalife-threateningdiseasewithlimitedtreatmentoptions.Inrecentyears,nanomedicinehasemergedasapromisingapproachforcancertherapy.Inthisstudy,weaimedtosynthesizetargetednanovesiclesusingnanobodytechnologyandevaluatetheirtargetingefficacyinrenalcancer.
Introduction:
Renalcancer,particularlyrenalcellcarcinoma(RCC),accountsforasignificantproportionofcancer-relateddeathsworldwide.Conventionaltreatments,suchassurgeryandchemotherapy,oftenhavelimitedefficacyandseveresideeffects.Nanomedicine,arapidlyevolvingfield,offersnewopportunitiesfortargetedtherapy.Amongthevariousnanoscalecarriers,nanovesicleshavegainedattentionduetotheiruniqueproperties,includinghighdrug-loadingcapacity,prolongedcirculationtime,andabilitytotargetspecificcelltypes.Additionally,nanobodies,whicharesingle-domainantibodiesderivedfromcamelids,haveshownexcellenttargetingpotentialduetotheirsmallsize,highspecificity,andlowimmunogenicity.Therefore,inthisstudy,weaimedtodevelopnanovesiclestargetedbynanobodiesandevaluatetheirpotentialforrenalcancertherapy.
Methods:
1.Isolationandselectionofnanobodies:
-Immunizationofcamelidswithrenalcancerantigenstogenerateantibodyresponse.
-Generationofphagedisplaynanobodylibraryfromtheperipheralbloodlymphocytes.
-Selectionofhigh-affinitynanobodiesagainstrenalcancercellsthroughmultipleroundsofpanning.
2.Preparationoftargetednanovesicles:
-Synthesisofliposomesorothersuitablenanovesicleformulationswithencapsulationofchemotherapeuticdrugsordiagnosticagents.
-Conjugationofselectednanobodiesontothesurfaceofnanovesiclesthroughappropriatechemicalmodificationstrategies.
3.Characterizationoftargetednanovesicles:
-Evaluationofsize,surfacecharge,drugencapsulationefficiency,andstability.
-Confirmationofnanobodyconjugationandretentionoftargetingspecificity.
4.Invitrovalidationoftargetingefficacy:
-Incubationoftargetednanovesicleswithrenalcancercellsandnon-cancerouscontr