(毕业论文)菊苣根中多糖的提取工艺.doc

长春工业大学学士毕业论文摘要本论文以菊苣根为原料，经过浸提、离心、过滤、除蛋白、浓缩、醇沉等工艺制备菊苣根多糖，试验分析比较了热水浴浸提法、醇浴浸提法、超声波水提法和超声波醇提法四种方法对菊苣根多糖的提取效果，并以菊苣根多糖提取率为考察对象，通过单因素试验分析，考察了提取温度、时间、料液比对多糖提取率的影响，确定Sevag法除蛋白中氯仿：正丁醇的最佳比例为3:1（v：v），醇沉时料液比为2：1（v：v），通过L9(34)正交实验，确定热水浴浸提法提取菊苣根多糖的最佳工艺条件为：提取温度为80℃，料液比1:20，提取时间为8h，在此最优条件下，菊苣根多糖的提取率达到6.426%。方法学考察表明，苯酚硫酸法在测定菊苣根多糖含量时，方法简便，结果准确，重现性好，稳定性好。关键词：菊苣根多糖；热水浴浸提法；提取工艺；正交试验AbstractChicory,thepaperasrawmaterial,afterextraction,centrifugationandfiltration,thepreparationofchicoryrootpolysaccharideinadditiontoproteinconcentration,andalcoholprecipitationprocess,testanalysisandcomparisonofthehotwaterbathextraction,alcoholbathextraction,ultrasonicwaterextractionandultrasonicethanolextractionfourmethodstheeffectoftheextractofchicoryrootpolysaccharide,polysaccharides,andchicoryrootextractwasinvestigatedobjectbysinglefactortestanalysis,theeffectsofextractiontemperature,time,solid-liquidratioofpolysaccharideextractionratetodeterminetheSevagmethodinadditiontoproteinchloroform:butanolratioof3:1(v:v),andalcoholprecipitationofsolidtoliquidratioof2:1(v:v),L9(34)orthogonalexperimentstodeterminetheoptimumconditionsforextractionofthehotwaterbathpolysaccharideextractofchicoryrootasfollows:extractiontemperatureof80°C,theratio1:20,extractiontimeof8h,theoptimalconditions,thepolysaccharideofchicoryrootextractrateof6.006%.MethodologicalstudyshowedthatthephenolsulfuricacidmethodinthedeterminationofChicoryrootpolysaccharidecontent,themethodissimple,accuratewithgoodreproducibility,goodstability.Keywords:Chicoryrootpolysaccharide；polysoccharide；extract；orthogonaltest；目录TOC\o"1-3"\h\z\uHYPERLINK\l"_Toc327685107"摘要PAGEREF_Toc327685107\hIHYPERLINK\l"_Toc327685109"AbstractPAGEREF_Toc327685109\hIIHYPERLINK\l"_Toc327685110"第一章前言PAGEREF_Toc327685110\h1HYPERLINK\l"_Toc327685112"第二章文献综述PAGEREF_Toc327685112\h1HYPERLINK\l"_Toc327685113"2.1菊苣根研究现状PAGEREF_Toc327685113\h1HYPERLINK\l"_Toc327685114"2.2菊苣根的功能PAGEREF_Toc327685114\h2HYPERLINK\l"_Toc327685115"2.2.1抗衰老作用P