庄少萍 灵芝液体发酵天然培养基响应面优化研究.docx

摘要灵芝是我国医药宝库中一种珍贵滋补中药材，具有很好的保健作用及很高的药用价值。液体深层发酵技术是一种具有较大开发利用前景的灵芝生产方法。本实验利用液体深层发酵技术，与传统的半合成培养基进行对比，观察灵芝菌丝生长状况及多糖含量的变化，设计了一种选用可食用原料按比例配制的液体天然培养基。结果表明：液体天然培养基配比为蔗糖40g/L，豆粉26g/L，钙镁片1.60g/L，氯化钾0.50g/L，灵芝菌丝生长旺盛，灵芝生物量为16.0g/L，胞外多糖含量为1.91g/L。关键词：灵芝；液体深层发酵；液体天然培养基；多糖AbstractGanodermalucidumisakindofprecioustonicChineseherbalmedicine,Chinesemedicineithasverygoodhealthcarefunctionandmedicinalliquidsubmergedfermentationtechnologyofhighhavegreaterdevelopmentandutilizationprospectofganodermalucidumisakindofproductionmethodofthisexperimentusingliquidsubmergedfermentationtechnology,comparedwiththetraditionalsemisyntheticmedium,thenobservethechangesofganodermalucidumhyphagrowthandpolysaccharidecontent,designakindofnaturalediblerawmaterialsaccordingtotheproportionoftheliquidculturemedium.Theresultsshowedthat:theproportionofliquidnaturalmediumwassucrose40g/L,soybeanpowder26g/L,calciumandmagnesiumtablets1.60g/L,potassiumchloride0.50g/L,thegrowthofganodermalucidummyceliumwasvigorous,thebiomassofganodermalucidumwas16.0g/L,andthecontentofextracellularpolysaccharidewas1.91g/LKeywords：Ganodermalucidum;Liquidfermentation;Liquidnaturalmedium;polysaccharidePAGE\*MERGEFORMAT19目录HYPERLINK\l"_Toc30434_WPSOffice_Level1"1引言2HYPERLINK\l"_Toc30434_WPSOffice_Level2"1.1灵芝概述2HYPERLINK\l"_Toc15262_WPSOffice_Level2"1.2灵芝保健功能及价值2HYPERLINK\l"_Toc1569_WPSOffice_Level2"1.3灵芝主要活性成分3HYPERLINK\l"_Toc30434_WPSOffice_Level3"1.3.1灵芝多糖3HYPERLINK\l"_Toc15262_WPSOffice_Level3"1.3.2三萜类化合物3HYPERLINK\l"_Toc1569_WPSOffice_Level3"1.3.3灵芝蛋白质和氨基酸3HYPERLINK\l"_Toc32348_WPSOffice_Level2"1.4灵芝液体深层发酵4HYPERLINK\l"_Toc9494_WPSOffice_Level2"1.5研究背景及意义4HYPERLINK\l"_Toc15262_WPSOffice_Level1"2材料和方法5HYPERLINK\l"_Toc23106_WPSOffice_Level2"2.1材料、药品