茶树菇废料对滑菇漆酶活性变化规律的影响 毕业论文.docx

3摘要在生产完毕的茶树菇废料中仍含有大量的有机物质和各种营养成分为探索滑菇菌种在液体培养基的不同茶树菇废料比例条件下对滑菇产生漆酶活性的影响利用ABTS（22-联氮-3-乙基-苯并噻唑-6-磺酸）的方法探讨茶树菇培养基废料比例对滑菇漆酶活性的影响。通过替代液体培养基的碳源和氮源分别设定为占总值的0%、20%、40%、60%、80%、100%。通过单因素实验方法主要是测定在相同培养基配方的不同废料比例的条件下滑菇菌丝每日的漆酶活性。通过单一变量实验研查废料比例对滑菇漆酶活性的影响可为以后废料利用研究提供参考。关键词：滑菇；培养基；微碱化；ABTS法；漆酶AbstractThewasteofediblemushroomproductionstillcontainsavarietyofnutrientsandalargenumberoforganicsubstances.InordertoexploretheinfluenceofshiitakemushroomonthelaccaseactivityofshiitakemushroomindifferentproportionofwasteofchashugumushroominliquidculturemediumtheinfluenceoftheproportionofwasteofchashugumushroomculturemediumonthelaccaseactivityofshiitakemushroomwasstudiedbyusingABTS(22-diazo-3-ethyl-benzothiazole-6-sulfonicacid).Thecarbonsourceweresetas0%20%40%60%80%and100%ofthetotalvaluerespectively.Bysinglefactorexperimentthelaccaseactivityofshiitakemyceliumwasdeterminedundertheconditionofdifferentwasteratioofthesamemedium.Theeffectofwasteproportiononlaccaseactivityofshiitakemushroomwasstudiedbysinglevariableexperimentwhichcanprovidereferenceforthefuturestudyofwasteutilization.Keywords:Shiitake;culturemedium;microalkalization;ABTSmethod;laccase目录1.引言............................................................11.2滑菇漆酶测定..................................................11.3漆酶..........................................................1材料与方法.....................................................32.2.1原料........................................................32.1.2仪器设备....................................................32.1.3药品........................................................32.2方法..........................................................42.2.1菌种活化以及扩种............................................42.2.2培养基的配置................................................42.2.3灭菌........................................................52.2.4接种及培养.............