阿托伐他汀对兔心肌缺血再灌注损伤的实验研究.docx

阿托伐他汀对兔心肌缺血再灌注损伤的实验研究[摘要]目的：研究阿托伐他汀（ATV）对心肌缺血再灌注损伤的影响及两种ATP依赖的K+（K+-ATP）通道的作用。方法：将健康家兔随机分为对照组、ATV组、ATV+5-HD（A1组）、ATV+HMR1883（A2组）。进行30min局部缺血和30min再灌注。实验中持续监测左室舒张末期压(LVEDP)、左心室压最大变化速率(±dp/dtmax)的变化再灌注结束后用伊文蓝和TTC染色法确定缺血和梗死心肌范围。结果：与对照组相比ATV组及A2组的梗死面积显著减少（P＜0.05）各项心功能指标在再灌注30min后明显改善（P＜0.05）；这种效应能够被5-HD所消除A1组与对照组相比无显著性差异（P＞0.05）。结论：ATV对心肌缺血再灌注损伤有早期保护效应且这种作用可能与心肌线粒体KATP通道有关系。[关键词]阿托伐他汀；心肌缺血再灌注损伤；ATP敏感性钾通道；保护效应[中图分类号]R961[文献标识码]A[文章编号]1673-7210（2009）06（b）-043-02Theeffectsofatorvastatinonthepreventionofheartmuscleischemia-reperfusioninjuryinrabbitsZHOUChangqingMAJie(DepartmentofCandiothoracicSurgerytheSecondTeachingHospitalofShanxiMedicalUniversityTaiyuan030001China)[Abstract]Objective:Toobservetheeffectofatorvastatin(ATV)onmyocardialischemia-reperfusioninjuryandtheroleoftwoATP-sensitiveK+channels．Methods:Thirty-tworabbitswererandomlydividedintofourgroupscontrolgroupATVgroupATV+5-HD(A1)groupATV+HMR1883(A2)group.Allrabbitsweresubjectedto30minischemiaand30minreperfusion.Themaxrateofriseofleftventricularpressure(±dp/dtmax)andleftventricularend-diastolicpressure(LVEDP)weredetectedduringtheexperiment.AttheendofreperfusiontheareaofnecrosisandareaofischemiaweredeterminedbyEvansblueandTTCstaining.Results:Comparedwiththecontrolgrouptheindexesofheartfunctionwereimprovedsignificantlyandinfarctsize(AN/AAR)werereduced(P＜0.05)inATVgroupandA2group.Andthiseffectwasblockedby5-HD.TherewasnostatisticallysignificantbetweenthecontrolgroupandA1group(P＞0.05).Conclusion:AtorvastatinexertsanearlycardioprotectiveeffectagainstmyocardialischemiareperfusioninrabbitsandmitochondrialKATPchannelislikelyinvolvedinthisprotectivemechanism.[Keywords]Atorvastatin;Myocardialischemia-reperfusion;ATP-sensitiveK+channels;Protectiveeffect随着医疗技术的发展各种治疗缺血性心脏病的方