针刺任脉和督脉对脑缺血大鼠海马星形胶质细胞的影响.docx

针刺任脉和督脉对脑缺血大鼠海马星形胶质细胞的影响[摘要]目的：研究针刺任脉和督脉经穴对局灶性脑缺血大鼠缺血海马星形胶质细胞的调节作用。方法：100只成年健康雄性Wistar大鼠随机分为假手术（sham）组、模型组、针刺督脉组及针刺任脉和督脉组sham组10只其余3组又分别随机分为7d组、14d组和28d组3个亚组每亚组10只。Wistar大鼠以线栓法制作大鼠大脑中动脉栓塞(MCAO)模型。通过免疫荧光双标法研究各组胶质纤维酸性蛋白(GFAP)阳性细胞和胶质纤维酸性蛋白/神经元特异性烯醇化酶(GFAP/NSE)双标细胞的表达。结果：针刺督脉治疗可以下调脑缺血后海马齿状回的GFAP阳性细胞数同时使GFAP/NSE双标细胞增多；针刺任脉和督脉治疗的GFAP阳性细胞增殖幅度小于针刺督脉组而GFAP/NSE双标细胞的增殖幅度则大于针刺督脉组。结论：针刺任脉和督脉可抑制脑缺血后海马星形胶质细胞的过度增殖并可促进细胞分化。[关键词]针刺；任脉；督脉；星形胶质细胞；大脑中动脉梗死[中图分类号]R742[文献标识码]A[文章编号]1673-7210（2008）11（a）-007-03TheeffectofelectroacupunctureatRenandDuvesselsonhippocamphorizontalcellsoffocalcerebralischemiaYANGZhuo-xin1YUHai-bo1LUOWen-shu1PIMin1DIAOLi-hong2RAOXiao-dan1YANGFu-xia1CAOXue-mei1(1.AcupunctureandMoxibustionWardShenzhenTCMHospitalShenzhen518033China;2.RehabilitationDepartmentShekouPeople'sHospitalShenzhen518000China)[Abstract]Objective:ToinvestigatetheregulatoryeffectsofelectroacupuncturingatRenandDuvesselsonhippocamphorizontalcellsoffocalcerebralischemia.Methods:100Wistarratswererandomlydividedintofourgroups:shamgroupMCAOgroupelectroacupuncturingatDuvesselgroupandelectroacupuncturingatRenandDuvesselsgrouptheshamgrouphad10ratstheotherthreegroupswerefurtherdividedintothreesubgroups:7dgroup14dgroupand28dgroupwitheachsubgroupof10ratsWistarratswereemployedtopreparethemiddlecerebralarteryocclusion(MCAO).Thenimmunofluorescencedouble-stainingmethodwasappliedtodetecttheexpressionsofglialfibrillaryacidicprotein(GFAP)positivecellsandglialfibrillaryacidicprotein/neuronspecificenolase(GFAP/NSE)double-immunostainingpositivecells.Results:ElectroacupuncturingDuvesselcoulddownregulatethenumberofGFAPpositivecellsofthehippocampaldentategyrusinfocalcerebralischemiaandincreasedthenumberofGFAP/NSEdouble-immunostainingpositivecells.TheproliferationofGFA