正交试验优化槟榔免煎颗粒提取工艺.docx

正交试验优化槟榔免煎颗粒提取工艺[摘要]目的优选槟榔免煎颗粒提取工艺。方法以出膏率和槟榔碱含量为指标采用正交试验和单因素试验考察槟榔药材浸泡时间、固液比、提取时间、提取次数对槟榔提取工艺的影响通过高效液相色谱法测定槟榔碱的含量SCX-强阳离子交换树脂柱（4.6mm×250mm5μm）；乙腈-磷酸溶液（21000）（70∶30）检测波长为215nm流速1mL/min进样量10μL。结果槟榔免煎颗粒的最优提取工艺为药材浸泡2h加6倍量水1h/次煎煮2次；平均出膏率为22.69%（RSD为1.67%）槟榔碱平均含量为4.63mg/g（RSD为1.82%）。结论优选的提取工艺稳定、可行为槟榔免煎颗粒的开发提供了实验基础。[关键词]槟榔；免煎颗粒；正交试验；提取工艺；单因素分析[中图分类号]R927.1[文献标识码]A[文章编号]1673-7210（2016）01（a）-0043-04Optimizationofextractionprocessforboil-freegranuleofarecacatechuLUNZhicai1SUIYing2XUGang3HUChaoqi3XUHuidan4CHENXin21.DepartmentofPharmacyJuxianHospitalofTraditionalChineseMedicineShandongProvinceRizhao276800China；2.SchoolofPharmaceuticalSciencesChangchunUniversityofChineseMedicineJilinProvinceChangchun130117China；3.QualityManagementDepartmentWuhuSimcereSino-implantPharmaceuticalCo.Ltd.AnhuiProvinceWuhu241080China；4.ProductionTechnologyDepartmentWuhuLUYEPharmaceuticalCo.Ltd.AnhuiProvinceWuhu241000China[Abstract]ObjectiveTooptimizeextractionprocessforboil-freegranuleofarecacatechu.MethodsWiththeextractionamountandcontentofarecolineasindexessinglefactoranalysismethodwasusedtodevelopsoaktimeofarecacatechuandeffectsofratioofsolid-liquidextractiontimesextractionquantityonextractiontechnologywereinvestigatedbyorthogonaltest.ThecontentofarecolinewasdeterminedbyHPLCSCX-strongcationchromatographiccolumn（4.6mm×250mm5μm）acetonitrile-phosphoricacid（21000）（70∶30）λ=215nmv=1mL/minsamplequantitywas10μL.ResultsThebestextractionprocessforboil-freegranuleofarecacatechuwasasfollowingsoaktimewas2hratioofsolid-liquidwas6∶1extractiontimewas1hextractionquantitywas1.Averageextractionratewas22.69%（RSD=1.67%）averagecontentofarecolinewas4.63mg/g（RSD=1.82%）.ConclusionThisoptimizedextractionprocessisstableandfeasiblew