川芎嗪对大鼠移植肝的保护作用.docx

川芎嗪对大鼠移植肝的保护作用作者:刘胜雄欧阳永忠汤明吴飞跃【摘要】目的探讨川芎嗪（TMP）对大鼠移植肝的保护作用。方法参照Delriviere和Kamada等方法建立大鼠肝移植模型将大鼠随机分为3组：A组（假手术组）、B组（肝移植对照组）和C组（TMP干预组）。采用全自动生化分析仪检测术后血清肝功能(AST、ALT、LDH)水平；应用免疫组化SP法结合病理图像分析仪测定肝组织中Bcl-2、Bax的表达情况；HE染色观察其病理形态学变化。结果（1）C组AST、ALT、LDH明显低于B组（P0.05）。（3）A组无明显病理形态改变B组术后肝组织出现明显的病理损害C组病理变化明显减轻。结论TMP可能通过上调Bcl-2或Bcl-2/Bax表达而减轻肝移植体的低温保存再灌注损伤对供肝有保护作用。【关键词】肝移植；保存再灌注损伤；川芎嗪；Bcl-2；BaxProtectiveeffectoftetramethylpyrazineonlivergraftinratlivertransplantation【Abstract】ObjectiveToinvestigatetheprotectiveeffectoftetramethylpyrazine(TMP)preconditioningonlivergraftinratlivertransplantation.MethodsPerformingtheoperationoflivertransplantationinmaleSprague-DawleyratsreferredtoDelriviereandKamadawithcuffanastomosingmethodstheratswererandomlydividedintothreegroups：Agroup(n=6shamoperationgroup)Bgroup(n=30×2livertransplantationcontrolgroup)Cgroup(n=30×2TMPgroup).Theparametersofliverfunction(ASTALTandLDH)weretestedbyauto-biochemicalanalyseringroupBandCafterreperfusion0.5h3h6h24h48hrespectivelyviatheplasmasamples；partofthelivertissuewassampledtodeterminetheexpressionofBcl-2andBax.TheparametersingroupAwereexamed3haftertheoperation.TheexpressionofBcl-2andBaxwasdeterminedbyimmunohistochemistytechniquewithpathologicalimaginganalyser.Alsopartsoflivertissuesampleswereusedtodetectthehistopathologicchanges.Results（1）ThelevelsofASTALTandLDHweresignificantlydecreasedingroupCcomparedwiththatingroupB（P＜0.01）afterreperfusion0.5h3h6h24h48hrespectively.（2）TheexpressionofBcl-2weresignificantlyincreasedingroupCcomparedwiththatingroupB（P＜0.01）afterreperfusion0.5h3h6hrespectively.ThedifferentexpressionofBaxbetweengroupCandgroupBwasnonsignificant.（3）ApproximatelynormallymorphologicstructurewasfoundingroupAsomeobvioushistopathologicchangesingroupBcouldbeseenmorpho