川芎嗪对大鼠心脏缺血-再灌注损伤的保护作用.docx

川芎嗪对大鼠心脏缺血-再灌注损伤的保护作用[摘要]目的：探讨川芎嗪对大鼠心肌缺血-再灌注损伤的保护作用。方法：SD大鼠30只分为假手术组、模型组和川芎嗪治疗组造模后分别于缺血30min、再灌注60min观察三组大鼠的血清CK、AST、LDH水平和心肌组织丙二醛（MDA）含量和超氧化物歧化酶（SOD）的活性变化。结果：模型组与对照组比较CK、AST、LDH指标升高心肌组织MDA含量和SOD活性升高同治疗组相比CK、AST、LDH水平升高心肌组织MDA含量升高SOD活性降低。结论：川芎嗪对大鼠心肌缺血-再灌注引起心肌损伤具有明显的保护作用其机制可能与抑制自由基生成和清除氧自由基作用有关。[关键词]川芎嗪；心肌缺血-再灌注损伤；丙二醛；超氧化物歧化酶[中图分类号]R285.5[文献标识码]A[文章编号]1673-7210（2009）03（c）-017-02ProtectiveeffectsofLigustrazineonmyocardialischemia-reperfusioninjuryinratsZHANGQunfengCHENLianjian*LIMin(DepartmentofPharmacytheFutianAffiliatedHospitalofGuangdongMedicalCollegeShenzhen518029China)[Abstract]Objective:TostudytheprotectiveeffectsofLigustrazineonmyocardialischemia-reperfusioninjuryinrats．Methods:30SDratswererandomlydividedintothreegroups:shamoperativegroup(SG)modelgroup(MG)andtreatmentgroup(LigustrazinegroupMG).Theactivitiesofcreatinkinase(CK)aspartateaminotransferase(AST)andlactatedehydrogenase(LDH)inserumweredeterminedandactivitiesofmalondialdehyde(MDA)andsuperoxidedismutase(SOD)aftermyocardialischemia30minandreperfusion60min.Results:comparedwithSGthelevelsofCKASTANFandLDHwereincreasedactivitiesofMDAandSODwereincreasedincardiacmuscleonMG.ComparedwithTGthelevelsofCKASTANFandLDHinserumwereincreasedactivityofMDAwasincreasedandthatofSODwasdecreasedincardiacmuscle.Conclusion:ProtectiveeffectsofLigustrazineonmyocardialischemia-reperfusioninjuryinratsisoutstandingwhichispossiblycorrelativewithresistingandclearingSOD.[Keywords]Ligustrazine;Myocardialischemia-reperfusioninjury;MDA;SOD1材料与方法1.1主要仪器与试剂超氧化物歧化酶检测试剂盒、丙二醛检测试剂盒、蛋白检测试剂盒、DH-140B型动物人工呼吸机、全自动生化测定仪、721型可见分光光度计。1.2动物分组与模型建立成年清洁级雄性SD大鼠30只重250～300g由南方大学医学院实验动物中心提供随机分为三组：假手术组、模型组、川芎嗪治疗组大鼠腹腔注射戊巴比妥钠麻醉（30mg/kg）经气管建立人工机械通气呼吸频率65～70次/min。分离左侧股静脉插管用于药物的滴注。于胸骨左缘2～4肋间打开胸腔及心包膜5-0号丝线