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响应面法优化龙眼多糖热水浸提的工艺.docx

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响应面法优化龙眼多糖热水浸提的工艺 Title:OptimizationofLonganPolysaccharideHot-WaterExtractionusingResponseSurfaceMethodology Abstract: Longanpolysaccharideisasignificantbioactivecompoundextractedfromthelonganfruit.Thisstudyaimedtooptimizethehot-waterextractionprocessoflonganpolysaccharideusingresponsesurfacemethodology(RSM).Theprocessvariables,includingextractiontemperature,extractiontime,andratioofwatertorawmaterial,wereselectedasindependentfactors.Theextractionyieldoflonganpolysaccharidewasusedastheresponsevariable.TheexperimentaldatawereanalyzedusingRSMtodevelopamathematicalmodelanddeterminetheoptimumconditionsforthehighestextractionyield.Theoptimizedprocesscancontributetotheefficientproductionoflonganpolysaccharidewithenhancedbioactivity. Keywords:Longanpolysaccharide,hot-waterextraction,optimization,responsesurfacemethodology 1.Introduction Longan(DimocarpuslonganLour.)isapopulartropicalfruitwithasignificanteconomicvalue.Apartfromitsrichnutritionalcontent,longanisknownforcontainingbioactivecompoundssuchaspolysaccharides,whichhaveshownvariousbeneficialhealtheffects,includingimmunomodulatory,antioxidant,andanti-tumoractivities(1).Theextractionoflonganpolysaccharidecanbeachievedviadifferentmethods,includinghot-waterextraction,enzyme-assistedextraction,andmicrowave-assistedextraction(2).Amongthesemethods,hot-waterextractioniswidelyusedduetoitssimplicity,lowcost,andenvironmentalfriendliness. 2.MaterialsandMethods 2.1.Materials Freshlonganfruitswereobtainedfromalocalmarketandwashedthoroughlybeforeuse.Allchemicalsandreagentsusedwereofanalyticalgrade. 2.2.Hot-waterextractionprocess Thehot-waterextractionoflonganpolysaccharidewasconductedusingaconventionalextractionapparatus.Theextractiontemperature,extractiontime,andratioofwatertorawmaterialwereselectedasindependentvariablesbasedonapreliminaryscreeningexperiment. 2.3.Responsesurfacemethodology(RSM) RSMisastatisticaltechniquewidelyusedintheoptimizationofvariousprocesses.Itinvolvesthedesignofexperiments,dataanalysis,andmathematicalmodel