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响应面法优化野生紫苏籽油提取工艺及其抗氧化活性.docx

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响应面法优化野生紫苏籽油提取工艺及其抗氧化活性 Title:OptimizationofExtractionProcessofWildPerillaSeedOilusingResponseSurfaceMethodologyanditsAntioxidantActivity Abstract: WildPerillaseedoilisknownforitsnumeroushealthbenefitsandhighantioxidantactivity.Optimizationoftheextractionprocessiscrucialtoobtainthemaximumyieldandqualityoftheoil.Inthisstudy,ResponseSurfaceMethodology(RSM)wasappliedtooptimizetheextractionconditionsofwildPerillaseedoilandevaluateitsantioxidantactivity. Keywords:WildPerillaseedoil,extractionprocess,responsesurfacemethodology,antioxidantactivity Introduction: WildPerilla(PerillafrutescensL.)isatraditionalmedicinalherbwidelyusedinEastAsiancountries.Itsseedsarerichinoil,withauniquefattyacidcompositionandabundantbioactivecompounds,includingphenoliccompounds,flavonoids,andtocopherols.TheoilextractedfromwildPerillaseedshasgainedincreasingattentionduetoitspotentialhealthbenefits,suchasanti-inflammatory,anti-allergenic,andanti-oxidativeactivities. Methods: 1.Materials:WildPerillaseedswereusedastherawmaterialforoilextraction.Solvents,includingethanol,n-hexane,andacetone,wereusedfortheextractionprocess. 2.Extractionprocess:TheextractionprocesswasoptimizedusingResponseSurfaceMethodology(RSM).RSMisastatisticaltechniquewidelyusedtooptimizethecombinationofseveralvariablesaffectingagivenresponse.Inthisstudy,fourvariables,includingextractiontime,extractiontemperature,solvent-to-materialratio,andsolventconcentration,wereevaluated.Acentralcompositedesign(CCD)wasusedfortheexperimentaldesign. 3.Oilyield:Theoilyieldwasdeterminedbygravimetricanalysisaftertheextractionprocess. 4.Characterization:ThewildPerillaseedoilwascharacterizedforitsphysicochemicalproperties,includingfattyacidcomposition,totalphenoliccontent,totalflavonoidcontent,andtocopherolcontent. 5.Antioxidantactivity:TheantioxidantactivityofthewildPerillaseedoilwasevaluatedusingvariousantioxidantassays,includingDPPHradicalscavengingactivity,reducingpowerassay,andlipidperoxidationinhibitionassay. ResultsandDiscussion: 1.Optimization