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响应面优化长白山紫苏籽油制备及其抗炎活性研究.docx

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响应面优化长白山紫苏籽油制备及其抗炎活性研究 Title:ResponseSurfaceOptimization,Preparation,andAnti-inflammatoryActivityofPerillaSeedOilfromChangbaiMountain Abstract: Perillaseedoil(PSO)isavaluablesourceofbioactivecompoundswithnumeroushealthbenefits,includinganti-inflammatoryproperties.ThisstudyaimedtooptimizethepreparationprocessofPSOfromPerillafrutescensseedscultivatedinChangbaiMountainusingresponsesurfacemethodology(RSM)andevaluateitsanti-inflammatoryactivity. Keywords:Perillaseedoil,responsesurfacemethodology,anti-inflammatoryactivity,bioactivecompounds,optimization. 1.Introduction Perillafrutescens,awidelygrownherbinAsia,isknownforitsnumerousmedicinalproperties.Theoilextractedfromitsseeds,commonlyknownasPerillaseedoil(PSO),isrichinpolyunsaturatedfattyacids(PUFAs),suchasalpha-linolenicacid(ALA).Theanti-inflammatoryactivityofPSOhasattractedconsiderableattention,makingitapotentialtherapeuticagentforinflammatorydiseases.However,thequalityandcompositionofPSOcanvarydependingonvariousfactors,includingcultivationconditionsandextractionmethods.Therefore,itisessentialtooptimizethepreparationprocesstoenhanceitsbioactiveproperties. 2.MaterialsandMethods 2.1ExperimentalDesign Responsesurfacemethodology(RSM)wasemployedtooptimizethepreparationprocessofPSO.Factorssuchasextractiontemperature,extractiontime,andsolvent-to-seedratiowereselectedasindependentvariables.Thedependentvariablesincludedoilyield,ALAcontent,andtotalphenoliccontent.ABox-Behnkendesign(BBD)wasusedfortheexperimentaldesign,andtheresponsedatawerefittedtosecond-orderpolynomialequations. 2.2OilExtractionandAnalysis PerillafrutescensseedswerecollectedfromChangbaiMountainanddried.PSOwasobtainedbysolventextractionusingaSoxhletapparatus.Theoilyieldwasdeterminedgravimetrically.ThecontentofALAwasdeterminedusinggaschromatography,andthetotalphenoliccontentwasmeasuredusingtheFolin-Ciocalteumethod. 2.3Anti-inflammatoryActivityEvaluation Theanti-inflammatoryactivityofPSOwasevaluatedusinginvitroandinvivomodels.Theinvitroassaysincludedtheinhibitionof