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健康奶牛子宫颈乳酸菌筛选方法的建立及其抑菌活性分析.docx

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健康奶牛子宫颈乳酸菌筛选方法的建立及其抑菌活性分析 Title:EstablishmentofaMethodfortheScreeningofLacticAcidBacteriafromHealthyCowCervixandAnalysisoftheirAntibacterialActivity Abstract: Thisstudyaimedtoestablishamethodforthescreeningoflacticacidbacteria(LAB)fromthehealthycowcervixandtoanalyzetheirantibacterialactivity.Thevaginalmicrobiotaplaysacrucialroleinmaintainingthereproductivehealthofcows.Lacticacidbacteriaareknownfortheirprobioticpropertiesandpotentialtoinhibitthegrowthofpathogenicbacteria.Therefore,theidentificationandcharacterizationofLABstrainsfromhealthycowcervixcancontributetothedevelopmentofnovelprobioticsforveterinarymedicine.Inthisstudy,weoptimizedaprotocolfortheisolationandidentificationofLABstrainsfromthecowcervixandevaluatedtheirantibacterialactivityagainstcommonpathogenicbacteria.TheresultsdemonstratedthepotentialofLABstrainsisolatedfromhealthycowcervixasprobioticsforpromotingreproductivehealthincows. Keywords:lacticacidbacteria,cowcervix,screeningmethod,antibacterialactivity 1.Introduction 1.1Importanceofreproductivehealthincows 1.2Roleoflacticacidbacteriainvaginalmicrobiota 1.3SignificanceofscreeningLABstrainsfromhealthycowcervix 2.MaterialsandMethods 2.1Samplecollection 2.2IsolationandidentificationofLABstrains 2.3OptimizationofgrowthconditionsforLABstrains 2.4Assessmentofantibacterialactivity 3.Results 3.1IsolationandidentificationofLABstrainsfromhealthycowcervix 3.2OptimizationofgrowthconditionsforLABstrains 3.3AntibacterialactivityofLABstrainsagainstcommonpathogens 4.Discussion 4.1SignificanceofLABstrainsisolatedfromhealthycowcervix 4.2FactorsaffectingthegrowthofLABstrains 4.3MechanismsofantibacterialactivityexhibitedbyLABstrains 5.Conclusion Thestudysuccessfullyestablishedamethodforthescreeningoflacticacidbacteriafromhealthycowcervixandevaluatedtheirantibacterialactivity.LABstrainsisolatedfromhealthycowcervixshowedpotentialasprobioticsforpromotingreproductivehealthincows.Furtherresearchiswarrantedtoelucidatethemolecularmechanismsunderlyingtheirantibacterialactivityan