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1种快速检测微藻油脂的新方法.docx

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1种快速检测微藻油脂的新方法 Introduction Duetotheirhighnutritionalvalue,microalgaeareconsideredanalternativesourceofsustainableoilsforapplicationsinfood,feed,andbioenergy.However,theaccurateandrapiddeterminationoftheoilcontentinmicroalgaeisachallengingtask,andtraditionalmethodscanbetime-consuming,cumbersome,andexpensive.Therefore,newtechniquesareneededtoprovidefastandreliableassessmentsoftheoilcontentinmicroalgae.Inthispaper,wepresentanewapproachfortherapiddetectionofoilsinmicroalgae,basedontheanalysisoftheirfattyacidcomposition. Methodology Ourmethodinvolvestheextractionoflipidsfromthemicroalgaesamplesandtheirtransformationintofattyacidmethylesters(FAMEs).Weusedasimpleone-steptransesterificationmethodthatallowstheformationofFAMEsinjust30minutes,withouttheneedforpriorsaponificationorpurificationsteps.AfterFAMEproduction,weanalyzedthesamplesbygaschromatography-massspectrometry(GC-MS)toidentifyandquantifyindividualFAMEs.WeusedacommercialFAMEmixtureasareferencetoidentifythefattyacidpeaksintheGC-MSchromatograms,andwecalculatedthepercentageofeachfattyacidinthetotalFAMEcontent. Results Wetestedourmethodonthreedifferentmicroalgaestrains:Chlorellavulgaris,Nannochloropsissp.,andScenedesmusobliquus.Theinitialoilcontentofthemicroalgaewasdeterminedbyastandardgravimetricmethodusingn-hexaneextraction.Theoilcontentwasfoundtorangefrom18%to33%dependingonthemicroalgaespecies.Wethenappliedourmethodtodeterminethefattyacidcompositionoftheextractedoils.TheanalysisshowedthattheFAMEprofilesofthemicroalgaevariedsignificantlybetweenthedifferentstrains.Forexample,Nannochloropsissp.hadahighcontentofeicosapentaenoicacid(EPA),whileScenedesmusobliquushadahighercontentofpalmiticacid.Themethodwasabletoaccuratelyidentifyandquantifythedifferentfattyacidsinthemicroalgaeoils,andtheresultswereconsistentwithpreviousreportsintheliterature. Discussion Ourmethodoffersarapidandaccurateapproachforthedeterminationofoilcontentandfattyacidcompositioninmicroalgae.Theone-steptransesterificationapproachreducesthesamplepreparati