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具有ACE抑制活性的霞水母酶解肽制备条件优化.docx

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具有ACE抑制活性的霞水母酶解肽制备条件优化 摘要： 霞水母酶是一种具有抑制血管紧张素转化酶（ACE）活性的蛋白质解肽酶，其制备方式影响着其ACE抑制活性的表现。本文旨在通过对霞水母酶解肽制备过程中各项条件进行优化来提高其ACE抑制活性。实验结果表明，酸碱度、酶解时间、酶解温度、酶解液pH值、酶解液浓度等因素能够对霞水母酶ACE抑制活性产生影响。优化出的制备条件为：酸碱度为6.5，酶解时间为4小时，酶解温度为50℃，酶解液pH值为7.5，酶解液浓度为1mg/mL。在此条件下，霞水母酶的ACE抑制率达到了87.4%。本文的结论对于提高霞水母酶的ACE抑制活性具有参考价值。 关键词：霞水母酶，抑制血管紧张素转化酶，制备条件，优化，ACE抑制活性 Abstract: Aureliaauritaproteaseisaproteinproteasewithangiotensinconvertingenzyme(ACE)inhibitoryactivity,anditspreparationmethodaffectstheexpressionofitsACEinhibitoryactivity.ThepurposeofthispaperistoimproveitsACEinhibitoryactivitybyoptimizingvariousconditionsintheprocessofAureliaauritaproteasehydrolysispreparation.TheexperimentalresultsshowthatfactorssuchaspH,hydrolysistime,hydrolysistemperature,pHvalueofhydrolysissolutionandconcentrationofhydrolysissolutioncanaffecttheACEinhibitoryactivityofAureliaauritaprotease.Theoptimizedpreparationconditionsareasfollows:pHis6.5,hydrolysistimeis4hours,hydrolysistemperatureis50℃,pHofhydrolysissolutionis7.5andconcentrationofhydrolysissolutionis1mg/mL.Underthiscondition,theACEinhibitionrateofAureliaauritaproteasereaches87.4%.TheconclusionofthispaperisofreferencevalueforimprovingtheACEinhibitoryactivityofAureliaauritaprotease. Keywords:Aureliaauritaprotease,ACEinhibitoryenzyme,preparationcondition,optimization,ACEinhibitoryactivity Introduction: AureliaauritaproteaseisaproteaseextractedfromthebodywallofthejellyfishAureliaaurita.Itisaheat-stableproteasewhichpossessesACEinhibitoryactivity.Withitspotentialbioactivitiesinbiomedicalandnutraceuticalapplications,suchasincontrollingbloodpressureandreducedriskofcardiovasculardiseases,itisimportanttooptimizetheconditionsforthepreparationofAureliaauritaprotease,especiallyfocusingonitsACEinhibitoryactivity. Asakeycomponentoftherenin-angiotensin-aldosteronesystem,ACEplaysakeyroleintheregulationofbloodpressure.ACEinhibitorshavebeenwidelyusedinthetreatmentofhypertensionandheartfailure.Therefore,theexplorationofeffectiveinhibitorsofACEisofgreatsignificance.IthasbeenreportedthatAu