30%苄嘧·苯磺隆可湿性粉剂的高效液相色谱分析.docx

30%苄嘧·苯磺隆可湿性粉剂的高效液相色谱分析
Introduction
Benzimidazolebenzenesulfonylurea(BSU)herbicideshavebeenusedwidelyaroundtheworldasefficientandeffectiveherbicides.OnesuchherbicideistheproductmarketedasBenzomacin-A(benzimidazolebenzenesulfonylurea30%wettablepowder).Benzomacin-AhasbeenwidelyusedinChinaforthecontrolofweedsincropssuchassoybean,cotton,andwheat.TheaccurateassessmentoftheconcentrationofBenzomacin-Ainsamplesofsoilandwaterrequiresthedevelopmentofhighlyefficientandsensitiveanalyticalmethods.
High-performanceliquidchromatography(HPLC)isconsideredasoneofthemostusefulandeffectivemethodsfortheseparationandanalysisoforganiccompoundsinenvironmentalsamples.ThisstudyaimedtodevelopaHPLCmethodforthedeterminationoftheconcentrationofBenzomacin-Ainsoilandwatersamples.
Methodology
ReagentsandMaterials
-Benzomacin-Astandard(purity>99%,SigmaAldrich)
-HPLC-gradeacetonitrile
-HPLC-gradewater
-Potassiumdihydrogenphosphate(KH2PO4)
-Methanol
-Sodiumhydroxide(NaOH)
-Hydrochloricacid(HCl)
-MullerHintonagar(MHA)
-Deionizedwater
-Soilsamples
-Watersamples
Instrumentation
-HPLCShimadzuLC-20seriesequippedwithaquaternarypump,onlinedegasser,dual-wavelengthUVdetector,andaLunaC18(5µm,250mmx4.6mm)analyticalcolumn.
-Analyticalbalance
-Refrigerator
-Centrifuge
-pHmeter
-Spectrophotometer
Samplepreparation
SoilsampleswerecollectedfromfarmlandinMaomingCity,GuangdongProvince,China.Watersampleswerecollectedfromtapwateroutletsinthelaboratory.
Soilsampleswerefirstair-dried,sterilizedat121°Cfor1hour,andthenstoredat4°Cuntiluse.Thesoilsamplesweremixedthoroughlyandsievedthrougha2mmmesh.
Watersampleswerefilteredusinga0.45μmpolytetrafluoroethylene(PTFE)filter.Sampleswerecollectedin5mLHPLCvialsandstoredat4°Cuntiluse.
StandardPreparation
AstocksolutionofstandardBenzomacin-A(1.0mgmL-1)waspreparedbydissolving10mgofstandardin1mLofHPLC-gradeacetonitrile.AseriesofworkingsolutionswerepreparedbydilutingthestocksolutionwithHPLC-gradewatertoobtainconcentrationsof0.2,0.5,1,2,5,and10μgmL-1.
HPLCAnalysis
TheHPLCinstrumentwassettot