预览加载中，请您耐心等待几秒...

UASB处理模拟酸性矿山废水后的污泥菌种分析.docx

预览
1/2
2/2

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

UASB处理模拟酸性矿山废水后的污泥菌种分析 Title:MicrobialAnalysisofSludgeafterUASBTreatmentofAcidicMiningWastewater Introduction: Acidicminingwastewaterisamajorenvironmentalconcernduetoitshighcontentofheavymetalsandacidity,whichcanresultinsevereecologicaldamage.TheUpflowAnaerobicSludgeBlanket(UASB)processisaneffectivemethodfortreatingacidicminingwastewater.ThisstudyaimstoanalyzethemicrobialcompositionofthesludgegeneratedafterUASBtreatmenttounderstandthemicroorganisms'roleinthetreatmentprocess. Methods: 1.SampleCollection:SludgesampleswerecollectedfromtheUASBreactorataminingwastewatertreatmentplant. 2.DNAExtraction:TotalmicrobialDNAwasextractedfromthesludgesamplesusingacommerciallyavailableDNAextractionkit. 3.PolymeraseChainReaction(PCR):TheextractedDNAwasamplifiedusinguniversalprimerstargetingthe16SrRNAgene,awidelyusedmarkerformicrobialcommunityanalysis. 4.Sequencing:TheamplifiedDNAwassubjectedtonext-generationsequencing(NGS)usingIlluminasequencingtechnologytoobtainhigh-throughputsequencedata. 5.BioinformaticsAnalysis:TheobtainedDNAsequenceswereanalyzedusingbioinformaticstoolsanddatabasestoidentifythemicrobialtaxapresentinthesludgesamples. 6.StatisticalAnalysis:Statisticalanalysiswasperformedtodeterminethediversityandabundanceofmicrobialspeciesinthesludgesamples. ResultsandDiscussion: Theanalysisofthemicrobialcompositionofthesludgesamplesrevealedadiversecommunityofmicroorganisms.ThedominantbacterialphylaidentifiedwereProteobacteria,Firmicutes,andBacteroidetes.Thesephylaareknowntoplayessentialrolesinwastewatertreatmentprocesses.WithinProteobacteria,Acidithiobacillus,Leptospirillum,andFerrovumwerethemostabundantgenera,whichareinvolvedinsulfurandironoxidation,keyprocessesinneutralizingacidicminingwastewater. Furthermore,severalgenerainvolvedinthebiologicalremovalofheavymetalswereidentified,suchasMagnetospirillum,Bacillus,andPseudomonas.Thesemicroorganismsareknowntopossessmetal-bindingcapabilitiesandcancontributetotheremovalofheavymetalsfromwastewater. Thestudyalsorevealedthepresenceofmet