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愈创木酚生产菌.docx

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Thatis,thepresentinventionprovidesthefollowingdetectionmethods(1)to(6),detectionkits(7)to(13),andmethodsforrapiddeterminationofproliferationproperties(14)to(17).(1)Amethodfordetectingbacteriacapableofproducingguaiacol,whichcomprisesculturingorincubatingaculturesolutioncontainingaspecimenforapredeterminedtimeinthepresenceofvanillicacid,andmeasuringtheproducedguaiacolbyqualitativeorquantitativeanalysis.(2)Themethoddescribedin(1),whereinsaidproducedguaiacolismeasuredbyGC-MSanalysisorcolorreaction.(3)Themethoddescribedin(1)or(2),whereinthecolorreactionforguaiacolemploysanoxidationreactionusingperoxidaseasacatalystandisdetectedbyspectrophotometryorvisualobservation.(4)Themethoddescribedinanyoneof(1)to(3),whereinsaidbacteriacapableofproducingguaiacoltobedetectedarethermotolerantacidophilicbacteriaandareculturedorincubatedunderanacidcondition.(5)Themethoddescribedinanyoneof(1)to(3),whereinsaidbacteriacapableofproducingguaiacoltobedetectedarebacteriabelongingtothegenusBacillusandareculturedorincubatedunderaneutralcondition.(6)Themethoddescribedin(4),whereinsaidthermotolerantacidophilicbacteriaareofA.acidoterrestris.(7)Akitfordetectionofbacteriacapableofproducingguaiacol,comprisingacontainer,andanacidorneutralculturemediumcontainingavanillicacidsubstrateforproductionofguaiacol,whichiscontainedinthecontainerandalsosterilizedandsealed.(8)Akitfordetectionofbacteriacapableofproducingguaiacol,comprisingacombinationofthekitdescribedin(7)andameansfordetectionofguaiacolincombination.(9)Thekitdescribedin(7)or(8),whereinsaidmeansfordetectionofguaiacolisbasedoncolorreactionandincludesacombinationofatleasthydrogenperoxidewater,aperoxidaseenzymeandabufferforenzymaticreaction.(10)Thekitfordetectionofbacteriacapableofproducingguaiacoldescribedin(9),comprisingalloranycombinationofthekitdescribedin(7),avanillicacidsubstrate,hydrogenperoxidewater,peroxidase,abuffersolution,afilterforfiltratingaculturesolution,amanualpumpforfiltratingaculturesolution,atransparentcellcontainingaculturefiltratea