甘蓝抗根肿病种质资源鉴定及抗性相关基因挖掘.docx

甘蓝抗根肿病种质资源鉴定及抗性相关基因挖掘
Title:IdentificationofCabbageGermplasmResourcesforResistancetoRootTumorDiseaseandExplorationofRelevantResistance-relatedGenes
Abstract:
Cabbage(Brassicaoleraceavar.capitata)isanimportantvegetablecropwidelycultivatedworldwide.Roottumordisease,causedbysoil-bornepathogens,posesasignificantthreattocabbageproduction,leadingtosignificantyieldlosses.Developingcabbagevarietieswithresistancetoroottumordiseaseiscrucialforsustainablecabbageproduction.Inthisstudy,weperformedacomprehensiveevaluationofcabbagegermplasmresourcesforresistancetoroottumordiseaseandexploredtheunderlyingresistance-relatedgenes.
Introduction:
Cabbageissusceptibletovarioussoil-bornepathogensthatcauseroottumordisease,includingPlasmodiophorabrassicae,Agrobacteriumrhizogenes,andRhizoctoniasolani.Thesepathogensinfectcabbageroots,leadingtotheformationofgalls,deterioratedrootsystems,andimpairednutrientuptake.Asaresult,theoverallcabbageplantgrowthishindered,culminatinginreducedyieldandquality.Breedingcabbagevarietieswithresistancetoroottumordiseaseisaneffectiveandsustainableapproachtomanagethisdisease.Theidentificationofcabbagegermplasmresourceswithnaturalresistanceandtheexplorationofresistance-relatedgenesarefundamentalstepstowardbreedingresistantcultivars.
Methods:
1.Collectionandmaintenanceofcabbagegermplasmresources
2.Artificialinoculationwithroottumordiseasepathogens
3.Phenotypicevaluationofresistancelevel
4.Molecularanalysisofresistance-relatedgenes
5.Validationofcandidategenesthroughfunctionalandassociationstudies
Results:
1.Identificationofcabbagegermplasmresourceswithvaryingresistancelevelstoroottumordisease
2.Characterizationofphenotypicdifferencesamongresistantandsusceptiblecabbagevarieties
3.Discoveryofcandidateresistance-relatedgenesthroughexpressionprofilingandcomparativegenomics
4.Validationofcandidategenesusingfunctionalgeneknockoutsandassociationstudies
5.Genomicselectionofresistantcabbagevarietiesforfuturebreedingprograms
Discussion:
Theevaluationofcabbagegermplasmresource