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NewYorkScienceJournal2015;8(2)http://www.sciencepub.net/newyork TheRapidPropagationTechniqueoftheMedicinalPlantClinacanthusnutansbyTissueCulture BihuaChen1,2,3,JuanZhang1,2,CuiZhang3,YumeiXiao3 1.FujianAcademyofForestrySciences,Fuzhou350012,China 2.KeyLaboratoryofTimberForestBreedingandCultivationforMountainousAreasinSouthernChina,Fuzhou 350012,China 3.FujianQingliuCountyForestryBureau,Qingliu365300,China chenbihua@hotmail.com Abstract:ThetraditionalmedicinalplantClinacanthusnutansisusuallypropagatedbycuttingpropagationwhich haslowreproductivecapacity.ThedevelopmentofrapidpropagationmethodsforC.nutansisneededtosatisfy humandemandforitsmedicinalproducts.Thisstudydevelopedapraticaltissueculturemicropropagationtechnique forC.nutansbyshootinitiationinsteadofcallusinduction.Theresultsshowedtheoptimalproliferationmediumfor C.nutanswasMurashigeandSkoog(MS)with1.0mgL-1BA+0.02mgL-1NAAproviding3.9multiplicationrate. Arootingmediumcomposedof½MS+0.25mgL-1IBAprovided100%rootingandvigourousplantlets.These methodsprovidereliablemass-propagationofC.nutansformedicinalpurposes. [ChenB,ZhangJ,ZhangW,ZhangC,XiaoY.TheRapidPropagationTechniqueoftheMedicinalPlant ClinacanthusnutansbyTissueCulture.NYSciJ2015;8(2):23-27].(ISSN:1554-0200). http://www.sciencepub.net/newyork.4 Keywords:Clinacanthusnutans(Burm.f.)Lindau;tissueculture;medium;plantgrowthregulator Abbreviation:BA-benzyladenine;NAA-naphthaleneaceticacid;IBA-indolebutyricacid 1.IntroductionbioactivechemicalconstituentsinChinasuggesting Clinacanthusnutans(Burm.f.)Lindau(namelythatC.nutanswasofhighnutritionalvalue(Yietal., SabahSnakeGrass)isaperennialherbbelongingto2012). thefamilyAcanthaceae,widelyknownasatraditionalInChina,therareC.nutansisusuallypropagated medicinalplantinSoutheastAisasuchasMalaysia,bycuttingpropagation,whichislowproliferation ThailandandChina(Guangxi,Guangdong,Yunnan,freqency.InMalaysia,Ying(2013)andGunasekaran andHainanProvince)duetoitsmedicinalpropertiesin(2014)succeededincallusinductionbutnoresultin trea