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具有潜在益生性的乳酸菌的筛选及其拮抗沙门氏菌黏附Caco--2细胞的机理研究.docx

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具有潜在益生性的乳酸菌的筛选及其拮抗沙门氏菌黏附Caco--2细胞的机理研究 Title:ScreeningofPotentialProbioticLacticAcidBacteriaandMechanisticStudyoftheirAntagonisticEffectonSalmonellaAdhesiontoCaco-2Cells Abstract: Probioticlacticacidbacteria(LAB)havegainedincreasingattentionduetotheirpotentialhealthbenefits.Inthisstudy,weaimedtoscreenLABstrainswithpotentialprobioticpropertiesandinvestigatetheirmechanismofactionagainstSalmonellaadhesiontoCaco-2cells.SeveralLABstrainswereisolatedfromdifferentsourcesandevaluatedfortheirprobioticcharacteristics,includingacidandbilesalttolerance,antimicrobialactivity,andadhesionability.Additionally,theantagonisticeffectoftheselectedLABstrainsagainstSalmonellaadhesiontoCaco-2cellswasassessed. Introduction: Probioticsarelivemicroorganismsthatconferhealthbenefitstothehostwhenadministeredinadequatequantities.Amongprobiotics,LABareacommonchoiceduetotheirinherentsafety,beneficialeffectsinmaintainingguthealth,andpotentialforantagonisticeffectsagainstpathogenicbacteria.Salmonellaisaleadingcauseoffoodborneinfections,anditsadhesiontointestinalepithelialcellsisacrucialstepinthepathogenesisofdisease.Therefore,understandinghowselectedLABstrainsinterferewithSalmonellaadhesiontoCaco-2cellscancontributetothedevelopmentofeffectivestrategiesforthepreventionandtreatmentofSalmonellainfections. Methods: LABstrainswereisolatedfromvarioussources,suchasfermentedfoodsandthegastrointestinaltractofhealthyindividuals.Theisolatedstrainswerethensubjectedtophenotypiccharacterization,includingacidandbilesalttolerance,antimicrobialactivityagainstvariouspathogens,andadhesionabilitytoCaco-2cells.Basedonthesecharacteristics,themostpromisingLABstrainswereselectedforfurtherinvestigation. Inthemechanisticstudy,SalmonellaadhesiontoCaco-2cellswasassessedusingacellculturemodel.TheselectedLABstrainswereco-incubatedwithSalmonella,andtheadhesioncapacityofSalmonellatoCaco-2cellswasquantified.Additionally,potentialmechanismsinvolvedintheantagonisticeffectwereinvestigated,includingcompetitionforadhesionsites,productio