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三株放线菌活性次级代谢产物及Naphthacemycins糖基化作用的研究.docx

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三株放线菌活性次级代谢产物及Naphthacemycins糖基化作用的研究 Title:StudyontheSecondaryMetabolitesofThreeStrainsofActinobacteriaandtheGlycosylationofNaphthacemycins Abstract: ThispaperintroducesastudyonthesecondarymetabolitesproducedbythreestrainsofActinobacteriaandtheglycosylationofNaphthacemycins.Actinobacteriaareknownfortheirabilitytoproduceawiderangeofbioactivecompoundswithpharmaceuticalpotential.Naphthacemycins,aclassofantitumorantibiotics,havebeenfoundtohavepotentcytotoxicactivityagainstcancercells.ThisresearchaimstoinvestigatethesecondarymetabolitesproducedbythesethreestrainsofActinobacteriaandexploretheglycosylationofNaphthacemycins,whichmayenhancetheirbiologicalactivitiesandpharmacokineticproperties. Introduction: ActinobacteriaareagroupofGram-positivebacteriawithhighG+CcontentintheirDNA.Theyarewidelydistributedinvariousecologicalniches,includingsoil,marineenvironments,andplants.Actinobacteriaarewell-knownfortheirabilitytoproduceadiversearrayofsecondarymetaboliteswithremarkablebioactivities,suchasantibiotics,antivirals,anticanceragents,andimmunosuppressants.Thesemetabolitesareofgreatimportancetohumanhealthandhavebeenwidelyappliedinthepharmaceuticalindustry. NaphthacemycinsareauniqueclassofantitumorantibioticsproducedbyActinobacteria.TheyexhibitpotentcytotoxicactivityagainstcancercellsbyinducingDNAdamageandinhibitingtheactivityoftopoisomerases.However,theclinicalapplicationofnaphthacemycinsislimitedbytheirpoorsolubilityandpotentialtoxicity.Glycosylationisacommonmodificationprocessinnaturalproductbiosynthesisandhasbeenutilizedtoimprovethebioactivity,pharmacokinetics,andphysicochemicalpropertiesofnaturalproducts.Therefore,understandingtheglycosylationofnaphthacemycinsmayprovideinsightsintotheirenhancementandutilizationincancertreatment. Methods: Inthisstudy,threestrainsofActinobacteriawereisolatedfromsoilsamplesandcultivatedunderoptimizedconditions.Crudeextractswereobtainedandsubjectedtovariouschromatographictechniques,includingthin-layerchromatography(TLC),high-performanceliquidchro