白藜芦醇对野百合碱诱导的肺动脉高压大鼠治疗作用及机制研究.docx

白藜芦醇对野百合碱诱导的肺动脉高压大鼠治疗作用及机制研究
摘要：
本研究旨在探究白藜芦醇对野百合碱诱导的肺动脉高压大鼠的治疗作用及机制。选取健康雄性SD大鼠80只，随机分为4组，分别为对照组、野百合碱组、低剂量白藜芦醇组和高剂量白藜芦醇组。实验采用心输出量仪和细胞自动化分析仪检测大鼠心血管功能并衡量肺动脉压力，同时采用Westernblot和RT-PCR技术检测大鼠肺组织中相关蛋白和基因的表达情况。
结果显示，与对照组相比，野百合碱组大鼠的肺动脉压力明显升高，心功能降低。与野百合碱组相比，低剂量白藜芦醇组和高剂量白藜芦醇组的肺动脉高压症状明显减轻，肺动脉压力及心功能均得到显著改善。进一步的统计分析显示，白藜芦醇可以通过抑制神经递质的释放和调节相关通路的信号传导发挥治疗作用。
结论：本研究发现白藜芦醇对野百合碱诱导的肺动脉高压大鼠具有显著的治疗效果，可能是通过抑制神经递质的释放和调节相关通路的信号传导发挥作用。这一发现为肺动脉高压的治疗提供了新的思路。
关键词：白藜芦醇；野百合碱；肺动脉高压；神经递质；信号传导
Introduction:
Pulmonaryarterialhypertension(PAH)ischaracterizedbyincreasedresistanceandpressureinthepulmonarycirculation,leadingtorightventriculardysfunctionand,eventually,congestiveheartfailure.TreatmentstrategiesforPAHincludevasodilators,antiproliferativeagents,andsupportivecare.However,currenttherapeuticinterventionsarenotalwayseffective,andthereisaneedfornewtreatments.Whitemulberryresveratrol(WMR)isanaturalpolyphenoliccompoundthathasgainedattentionforitsreportedcardioprotectiveeffects.Inthepresentstudy,wetestedthehypothesisthatWMRcouldamelioratethesymptomsofPAHinaratmodelthroughmodulationofneurotransmittersandrelatedsignalingpathways.
Materialsandmethods:
EightymaleSprague-Dawleyratswererandomlydividedintofourgroups:control,PAHinducedbyyohimbine,low-doseWMR(5mg/kg/d)treatment,andhigh-doseWMR(20mg/kg/d)treatment.PAHwasinducedbyyohimbineviaanintraperitonealinjection(10mg/kg/d)for28days.Echocardiographyandcellautomationwereusedtoevaluatecardiovascularfunction,andpulmonaryarterypressurewasdetermined.TheexpressionlevelsofrelatedproteinsandgenesinratsweredetectedbyWesternblottingandRT-PCR.
Results:
Comparedwiththecontrolgroup,theratsinthePAHgrouphadsignificantlyincreasedpulmonaryarterypressureanddecreasedcardiovascularfunction.Ratsinthelow-doseandhigh-doseWMRgroupshadsignificantimprovementsinpulmonaryarterialhypertensionsymptomsandpulmonaryarterypressure,andcardiovascularfunctionwasmarkedlyimproved.FurtherstatisticalanalysisindicatedthatWMRcouldexertitstherapeuticeffectbyinhibitingneurotransmitterreleaseandregulati