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宿主细胞microRNA库调控甲型流感病毒复制的机制研究.docx

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宿主细胞microRNA库调控甲型流感病毒复制的机制研究 Title:MechanismsofHostCellmicroRNALibraryRegulationinControllingInfluenzaAVirusReplication Abstract: InfluenzaAvirus(IAV)remainsasignificantglobalhealthconcernduetoitsabilitytorapidlymutateandevadehostimmuneresponses.Hostcellshavedevelopedvariousantiviralstrategies,includingtheproductionofsmallnon-codingRNAscalledmicroRNAs(miRNAs).miRNAsplayacrucialroleinpost-transcriptionalgeneregulation,allowingforfine-tuningofgeneexpression.ThispaperaimstoinvestigatethemechanismsbywhichthehostcellmicroRNAlibraryregulatesIAVreplication. Introduction: InfluenzaAvirusrepresentsamajorglobalhealththreat,causingannualepidemicsandoccasionalpandemicswithsevereconsequences.ThehostresponsetoIAVinfectioninvolvesmultipleintricateprocesses,amongwhichmiRNAshaveemergedaskeyregulators.miRNAsareshortRNAmoleculesthatbindtotargetmRNAs,resultingintranslationalrepressionordegradation.RecentstudieshaveidentifiedawidearrayofhostcellmiRNAsthatcanmodulateIAVreplication,providinginsightsintopotentialtherapeuticapproaches. Methods: VariousexperimentalapproacheshavebeenemployedtoinvestigatetheroleofhostcellmiRNAsinregulatingIAVreplication.Theseincludehigh-throughputscreeningtechniques,suchasRNAsequencingandmicroarrayanalysis,toidentifydifferentiallyexpressedmiRNAsuponIAVinfection.FunctionalstudiesinvolvetheuseofmiRNAmimicsorinhibitorstoassesstheimpactonviralreplication.Additionally,bioinformaticstoolsareusedtoidentifypotentialmiRNAtargetgenesandpathways. Results: NumerousstudieshaverevealedthatspecificmiRNAsaredifferentiallyexpressedduringIAVinfection,indicatingtheirpotentialinvolvementinregulatingviralreplication.Forinstance,miR-146ahasbeenshowntonegativelyregulateIAVreplicationthroughtargetingviralnucleoprotein(NP)mRNA.Similarly,miR-323-3pinhibitsviralreplicationbytargetingtheviralpolymerasesubunitPB1mRNA.Ontheotherhand,somemiRNAs,suchasmiR-132,promoteIAVreplicationbysuppressinghostantiviralresponses. Mechanisms: ThemechanismsthroughwhichhostcellmiRNAsregulateIAVreplicationare