预览加载中，请您耐心等待几秒...

昆虫幼虫生产重组蛋白研究进展.docx

预览
1/3
2/3
3/3

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

昆虫幼虫生产重组蛋白研究进展 Title:ResearchProgressinInsectLarvaeProductionofRecombinantProteins Introduction: InsectsareoneofthemostdiverseandabundantgroupsoforganismsonEarth,withawiderangeofecologicalroles.Amonginsects,theirlarvalstages,commonlyknownascaterpillarsormaggots,playacrucialroleingrowthanddevelopmentpriortometamorphosis.Inrecentyears,therehasbeengrowinginterestinutilizinginsectlarvaefortheproductionofrecombinantproteinsduetotheirnumerousadvantagesovertraditionalproteinexpressionsystems.Thispaperaimstoprovideanoverviewoftheresearchprogressinthefieldofinsectlarvaeproductionofrecombinantproteins. AdvantagesofInsectLarvaeasProductionHosts: Insectlarvaeofferseveraladvantagesasproductionhostsforrecombinantproteins.Firstly,theyhaveahighbiomassyieldwithrapidgrowthrates,allowingforlarge-scaleproduction.Therelativelylowcostoflarvalproductioncomparedtootherexpressionsystemsisalsoanattractivefeature.Additionally,insectlarvaehaveasimpleandcost-effectiverearingstrategy,makingiteasiertomanageandcontroltheproductionprocess.Furthermore,insectshavespecialmechanismsforproteinfolding,post-translationalmodifications,andsecretion,whichcanresultintheproductionofcorrectlyfoldedandfunctionalrecombinantproteins. MethodsofProteinProductioninInsectLarvae: Variousmethodshavebeendevelopedforproducingrecombinantproteinsininsectlarvae.Onecommonlyusedapproachisthegenerationoftransgenicinsectlarvaethroughtheintegrationofforeigngenesintotheirgenomes.Transgenictechnologyallowsforstableandheritableexpressionofrecombinantgenesinthelarvae.Thismethodhasbeensuccessfullyusedtoproduceawiderangeofproteins,includingtherapeuticproteins,enzymes,andvaccines. Anotherpromisingmethodistheuseofbaculovirus-mediatedexpressionsystems.Baculovirusesareinsect-specificvirusesthatinfectawiderangeofinsecthosts,includingtheirlarvae.Recombinantbaculovirusescanbeengineeredtocarryandexpressforeigngenesininsectlarvae.Thismethodoffershighlevelsofproteinexpressionandhasbeenappliedtoproduceseveralcomplexproteins,suchasgrowthfactors,cy