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基于无性繁殖过程的蝙蝠蛾拟青霉循环发酵方法（英文）.docx

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基于无性繁殖过程的蝙蝠蛾拟青霉循环发酵方法（英文） Title:CirculationFermentationMethodofBatMothBeauveriaBassianaBasedonAsexualReproductionProcess Abstract: TheBeauveriabassianaisawell-knownentomopathogenicfunguswithawiderangeofhostinsectsandhasthepotentialtobedevelopedasanenvironmentallyfriendlybiopesticide.TheuseofBeauveriabassianainagriculturehasbeenlimitedbecauseofitslowproductionefficiencyandhighproductioncost.Inthispaper,weproposedacirculationfermentationmethodbasedontheasexualreproductionprocessofbatmothBeauveriabassianatoimprovetheproductionefficiencyandreducetheproductioncost.Themethodincludesoptimizingtheparametersoffermentation,separatingthesporesfromthemycelium,andrecyclingthemyceliumtothenextfermentationcycle.TheresultsshowedthatthecirculationfermentationmethodsignificantlyimprovedtheproductionefficiencyofBeauveriabassianacomparedtothetraditionalfermentationmethod,andthesporeyieldreached1.2×10^11CFU/mLundertheoptimalfermentationparameters.Thismethodhasthepotentialtobeappliedtolarge-scaleproductionofBeauveriabassianaandpromotethedevelopmentofbiopesticides. Keywords:Beauveriabassiana,circulationfermentation,asexualreproduction,biopesticide,productionefficiency Introduction: Withthecontinuousdevelopmentofagriculture,theuseofchemicalpesticideshasbecomewidespread,leadingtoenvironmentalpollutionandhealthrisks.Therefore,thedevelopmentofenvironmentallyfriendlybiopesticideshasbecomeanurgentissue.Beauveriabassianaisanentomopathogenicfungusthatcaninfectawiderangeofhostinsects,includingpestssuchascottonbollworm,tobaccobudworm,andbeetarmyworm.Ithasmanyadvantagescomparedtochemicalpesticides,includinglowtoxicity,highspecificity,andenvironmentalfriendliness,andhasthepotentialtobecomeanimportantbiopesticide.However,thelowproductionefficiencyandhighproductioncostlimitthecommercialapplicationofBeauveriabassiana. Inrecentyears,therehavebeenmanystudiesonthefermentationoptimizationofBeauveriabassiana,includingtheoptimizationoffermentationparameters,theuseofadditiveagents,andtheoptimizationoffermentat