预览加载中，请您耐心等待几秒...

芹菜素对非洲爪蟾卵母细胞体外成熟的影响及其作用机制研究.docx

预览
1/3
2/3
3/3

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

芹菜素对非洲爪蟾卵母细胞体外成熟的影响及其作用机制研究 Abstract: Apigeninisaflavonoidcompoundfoundinceleryandmanyotherplants,havingdiversebiologicalactivities.Inthisstudy,weinvestigatedtheeffectofapigeninoninvitromaturationofAfricanclawedfrog(Xenopuslaevis)oocytesandexploredtheunderlyingmechanisms.Ourresultsshowedthatapigeninatconcentrationsof10-50μMsignificantlyinhibitedthegerminalvesicle(GV)breakdown,firstpolarbodyextrusion,andmaturationofoocytesinadose-dependentmanner.Moreover,apigenintreatmentresultedinadecreaseintheexpressionofmaturation-promotingfactor(MPF)subunitCyclinB1andCdk1inoocytes.Wealsofoundthatapigenininhibitedoocytematurationbyaffectingtheactivityofthemitogen-activatedproteinkinase(MAPK)pathway,asevidencedbydecreasedphosphorylationlevelsofMAPKkinases(MEK)andextracellularsignal-regulatedkinase(ERK).Furthermore,apigenintreatmentresultedinDNAdamageandtheactivationofp53signalingpathway,whichmayalsoplayaroleinapigenin-inducedinhibitionofoocytematuration.Inconclusion,ourfindingssuggestthatapigeninimpairsoocytematurationthroughinhibitionoftheMPFcomplexandtheMAPKsignalingpathway,aswellasinducingDNAdamageandactivatingp53signalingpathway. Introduction: Apigenin(4',5,7-trihydroxyflavone)isaflavonoidcompoundthatbelongstotheflavonesubclassofflavonoids,whicharefoundinavarietyoffruits,vegetables,andherbs,includingcelery,parsley,chamomile,andthyme.Apigeninhasbeenshowntopossessawiderangeofbiologicalactivities,includingantioxidant,anti-inflammatory,anti-tumor,andanti-proliferativeeffects.Previousstudieshavedemonstratedthatapigeninisinvolvedinmanyimportantcellularprocesses,suchascellcycle,apoptosis,andDNArepair,throughregulatingvarioussignalingpathways. Oocytematurationisanimportantprocessinthedevelopmentoffemalegametes,whichconsistsofgerminalvesicle(GV)breakdown,chromatincondensation,andfirstpolarbodyextrusion.Oocytematurationisregulatedbycomplexmechanismsinvolvingseveralsignalingpathways,includingthematuration-promotingfactor(MPF)complex,whichiscomposedofCyclinB1andCyclin-dependentkinase1(Cdk1