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杨梅素及二氢杨梅素对HEK293细胞hKv1.5通道的抑制作用研究.docx

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杨梅素及二氢杨梅素对HEK293细胞hKv1.5通道的抑制作用研究 摘要： 本文旨在研究杨梅素及二氢杨梅素对HEK293细胞hKv1.5通道的抑制作用。通过整体细胞膜片钳技术记录hKv1.5通道电流，研究杨梅素及二氢杨梅素对其电流的抑制作用。结果显示，随着药物浓度的增加，杨梅素和二氢杨梅素均可显著抑制hKv1.5通道电流。此外，二氢杨梅素的抑制作用更为明显。综合上述结果，认为杨梅素及二氢杨梅素均具有抑制hKv1.5通道的作用，有望成为治疗相关心血管疾病的潜在药物。 关键词：杨梅素，二氢杨梅素，hKv1.5通道，抑制作用 Abstract： TheaimofthisstudyistoinvestigatetheinhibitoryeffectsofmyricetinanddihydromyricetinonhKv1.5channelinHEK293cells.Whole-cellpatchclamptechniquewasusedtorecordthehKv1.5channelcurrents,andtheinhibitoryeffectsofmyricetinanddihydromyricetinonthecurrentwerestudied.TheresultsshowedthatbothmyricetinanddihydromyricetincouldsignificantlyinhibitthehKv1.5channelcurrentwithincreasingdrugconcentration.Inaddition,theinhibitoryeffectofdihydromyricetinwasmoresignificant.Inconclusion,bothmyricetinanddihydromyricetinhaveinhibitoryeffectsonhKv1.5channel,andtheyhavethepotentialtobecomepotentialdrugsforthetreatmentofrelatedcardiovasculardiseases. Keywords:myricetin,dihydromyricetin,hKv1.5channel,inhibitoryeffect Introduction: Cardiovasculardiseaseistheleadingcauseofdeathworldwide,andismainlycausedbydysfunctionofcardiacionchannels.hKv1.5channelisamajorionchannelincardiovasculartissues,anditsdysfunctioniscloselyrelatedtocardiovasculardiseasessuchasarrhythmiaandheartfailure.Therefore,findingdrugsthatcanregulatetheactivityofhKv1.5channelhasimportantclinicalsignificance. Myricetinanddihydromyricetinareflavonoidcompoundsthatarewidelyfoundinvariousplants.Thesecompoundshaveawiderangeofpharmacologicaleffects,suchasanti-inflammatory,antioxidant,andantitumoractivities.Somestudieshavealsoshownthatthesecompoundshaveaneffectofregulatingionchannelsinthecardiovascularsystem,buttheireffectonhKv1.5channelhasnotbeenfullystudied. Therefore,thisstudyaimedtoexploretheinhibitoryeffectsofmyricetinanddihydromyricetinonhKv1.5channelinHEK293cells,andtoevaluatetheirfeasibilityaspotentialdrugsforthetreatmentofcardiovasculardiseases. Materialsandmethods: 1.Reagentsandsolutions: MyricetinanddihydromyricetinwerepurchasedfromSigma-Aldrich.Stocksolutionsw