树鼩组织的全长转录组与组织表达谱分析.docx

树鼩组织的全长转录组与组织表达谱分析
Title:TranscriptomeandTissueExpressionProfilingofTarsiers:AComprehensiveStudyontheTreeShrewOrganization
Abstract:
Understandingthetranscriptomeandtissueexpressionprofilesofanorganismiskeytounravelingitsfunctionalgenomics.Inthisstudy,weinvestigatethefull-lengthtranscriptomeandtissueexpressionpatternsoftreeshrews(Tupaiabelangeri),asmallarborealmammalbelongingtotheScandentiaorder.Throughthiscomprehensiveanalysis,weaimtogaininsightsintothegeneticandmolecularbasisofthetreeshreworganization.
Introduction:
Thetreeshrewisanemergingmodelorganismduetoitsclosephylogeneticrelationshipwithprimatesandasharedevolutionaryhistory.Recentadvancesinhigh-throughputsequencingtechnologieshaveprovidedanopportunitytoexplorethetranscriptomeandtissue-specificexpressionpatternsindetail.Thisstudyaimstounravelthegeneticandmolecularmechanismsgoverningthetreeshreworganizationbyanalyzingthefull-lengthtranscriptomeandtissueexpressionprofiles.
Methods:
RNAsampleswereextractedfromvarioustissuesofadulttreeshrews,includingthebrain,liver,heart,kidney,andspleen.ThetotalRNAwasenrichedformRNA,andfull-lengthcDNAlibrarieswereconstructedusingamodifiedprotocol.Thelibrariesweresequencedusinganext-generationsequencingplatformtoobtainhigh-qualityreads.Bioinformaticsanalysis,includingtranscriptomeassembly,annotation,anddifferentialexpressionanalysis,wasperformedusingstate-of-the-arttoolsanddatabases.
Results:
Thetranscriptomeassemblyyieldedacomprehensivecatalogoftranscripts,includingbothknownandnovelcodingandnon-codingtranscripts.Theannotationofthetranscriptomerevealedthefunctionalannotationandclassificationofprotein-codinggenesandnon-codingRNAs.Thedifferentialexpressionanalysisprovidedinsightsintotissue-specificgeneexpressionpatterns,revealinggenesthatarehighlyexpressedandgenesthatarespecifictocertaintissues.Thetissueexpressionprofilingunveiledtheuniqueexpressionsignaturesofvarioustissues,highlightingtheirfunctionalspecializationandpotentialroleinphysiologicalprocesses.
Discussion:
Thecomprehens