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基于Miseq测序分析海南阿宽蕉居群根际土壤细菌群落多样性.docx

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基于Miseq测序分析海南阿宽蕉居群根际土壤细菌群落多样性 Title:AnalysisofSoilBacterialDiversityintheRhizosphereofHainanAmorphophalluskonjacCommunitybasedonMiSeqSequencing Abstract: Therhizosphereistheregionofsoilinfluencedbyplantroots,hostingadiversecommunityofmicroorganisms.Inthisstudy,weaimedtoanalyzethebacterialdiversityintherhizosphereoftheHainanAmorphophalluskonjac(AK),aplantnativetoHainanprovince,China,usingMiSeqsequencing.SoilsampleswerecollectedfromtheAKcommunityandsubjectedtoDNAextractionandbacterial16SrRNAgeneamplification.TheamplifiedfragmentsweresequencedusingMiSeqplatformandanalyzedtodeterminethebacterialdiversityandcommunitycomposition.TheresultsshowedahighbacterialdiversityintherhizosphereoftheAKcommunity,providinginsightsintotheinteractionsbetweenrootsandthesoilmicrobiome. Introduction: Therhizosphereisadynamicmicroenvironmentsurroundingplantroots,characterizedbyhighmicrobialactivity.Theinteractionsbetweenplantsandtherhizospheremicrobiomehavebeenrecognizedtoplayacrucialroleinnutrientcycling,plantgrowthpromotion,anddiseasesuppression.Thediversityandcompositionofthebacterialcommunityintherhizosphereareinfluencedbyvariousfactors,includingplantspecies,soiltype,andenvironmentalconditions.UnderstandingtherhizospheremicrobialcommunityinAmorphophalluskonjachasimportantimplicationsforthesustainablemanagementofthiscrop. Methods: SoilsampleswerecollectedfromtherhizosphereoftheAKcommunityinHainan.DNAwasextractedfromthesoilsamplesusingacommercialkit.TheV4regionofthebacterial16SrRNAgenewasamplifiedusingprimersspecifictobacteria.TheamplifiedfragmentswerepurifiedandsequencedusingtheMiSeqplatform.Theobtainedsequenceswereprocessedusingbioinformaticstoolstoremovelow-qualityreads,chimeras,andnon-bacterialsequences.Theremaininghigh-qualitysequenceswereclusteredintooperationaltaxonomicunits(OTUs)basedonasequencesimilaritythreshold.TaxonomicclassificationwasperformedbycomparingtherepresentativesequencesofeachOTUagainstadatabaseofknownbacterialsequences. Results: AtotalofXXhigh-qualitysequenceswereob