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固态发酵基质影响红曲他汀与色素产量的研究.docx

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固态发酵基质影响红曲他汀与色素产量的研究 Title:TheImpactofSolid-StateFermentationSubstrateontheProductionofMonacolinKandPigmentsbyMonascuspurpureus Abstract: Solid-statefermentationiswidelyemployedintheproductionofbioactivecompoundsbyvariousmicrobialstrains.Thisstudyinvestigatestheinfluenceofdifferentsolid-statefermentationsubstratesontheproductionofMonacolinK(lovastatin)andpigmentsbyMonascuspurpureus.TheaimofthisresearchistooptimizethefermentationconditionsforenhancedyieldsofMonacolinKandpigmentsandexploretheirpotentialapplicationsinindustriessuchaspharmaceuticalsandfoodcolorants. Introduction: Monascuspurpureusisafilamentousfungusthathasbeenusedforcenturiesinthetraditionalpreparationofredyeastrice,afermentedriceproductwithvarioushealthbenefits.Thefungalstrainisknownforitsabilitytoproduceseveralsecondarymetabolites,includingMonacolinKandvariouspigments.MonacolinK,alsoknownaslovastatin,isanaturallyoccurringstatinthatiswidelyusedasananticholesterolmedication.Additionally,Monascuspigmentspossessantioxidant,antimicrobial,andanticanceractivities,makingthemofinterestforindustrialapplications. Methods: 1.Selectionofsolid-statefermentationsubstrates:Differentsubstrates,suchasrice,wheat,andcorn,wereevaluatedfortheirsuitabilityinsupportingMonascuspurpureusgrowthandsecondarymetaboliteproduction.Thesesubstratesweresterilized,inoculatedwithMonascusspores,andincubatedatoptimaltemperatureandhumidity. 2.Fermentationoptimization:Parameterssuchastemperature,moisturecontent,pH,andincubationtimewereoptimizedtoenhanceMonacolinKandpigmentyields.Statisticalmethods,suchasresponsesurfacemethodology(RSM),wereemployedtodeterminetheoptimalfermentationconditions. 3.Analyticaltechniques:High-performanceliquidchromatography(HPLC)wasusedtoquantifyMonacolinKproduction.Spectrophotometricanalysiswasemployedtomeasurethepigmentcontentandassesspigmentprofiles. Results: 1.SubstrateinfluenceonMonacolinKandpigmentproduction:Amongthetestedsubstrates,riceshowedthehighestMonacolinKyield,followedbywheatandcorn.Ricesubstratealsor