预览加载中，请您耐心等待几秒...

几株主栽灵芝菌株液体菌种培养基优化及其活力评价.docx

预览
1/2
2/2

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

几株主栽灵芝菌株液体菌种培养基优化及其活力评价 Title:OptimizationofLiquidCultureMediumforPrimaryCultivationofReishiMushroom(Ganodermalucidum)MyceliumandEvaluationofItsViability Abstract: Ganodermalucidum,commonlyknownasReishimushroom,isanimportantmedicinalmushroomwithvarioushealthbenefits.LiquidculturemediumisfrequentlyusedfortheprimarycultivationofReishimushroommyceliumtoobtainhigh-qualitymycelialbiomass.ThispaperaimstooptimizetheliquidculturemediumforReishimushroommyceliumcultivationandevaluatetheviabilityofthecultivatedmycelium. Introduction: Reishimushroomhasbeenusedforcenturiesintraditionalmedicineduetoitspotentialtherapeuticproperties.ThecultivationofReishimyceliumprovidesasustainableandcontrolledmethodforobtainingitsbioactivecompounds.LiquidculturemediumisaneffectiveapproachforcultivatingReishimushroommycelium,asitallowsfornutrient-richconditionssuitableformycelialgrowth.However,thecompositionoftheliquidculturemediumplaysavitalroleinmyceliumyieldandquality.Therefore,itisessentialtooptimizetheliquidculturemediumtoachievemaximummycelialbiomass. Methods: Inthisstudy,severalkeyfactorsaffectingtheliquidculturemediumformulationwillbeinvestigated,includingcarbonandnitrogensources,inorganicsalts,andgrowthfactors.Differentcombinationsandconcentrationsofthesecomponentswillbetestedusingafactorialdesignapproach.Thegrowthrate,mycelialbiomassyield,andnutritionalcompositionofReishimyceliumwillbeevaluated. Results: Basedontheresultsobtained,anoptimizedliquidculturemediumforReishimushroommyceliumcultivationwillbeproposed.Themediumcompositionthatdemonstratesthehighestmycelialbiomassyield,growthrate,andnutritionalcompositionwillbeselectedastheoptimalmedium.TheoptimizedmediumisexpectedtoprovidesufficientnutrientsandoptimalgrowthconditionsforReishimycelium,yieldinghigh-qualitymycelialbiomass. ViabilityEvaluation: TheviabilityofthecultivatedReishimyceliumwillbeassessedthroughvariousmethods.Cellviabilitystaining,suchastrypanblueexclusiontestorfluoresceindiacetate-ethidiumbromidestaining,willbeemployedtoe