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小麦抗性淀粉的遗传特性及其分子标记的筛选 Title:GeneticCharacteristicsandMolecularMarkersforWheatResistantStarch Abstract: Wheatisoneofthemostwidelycultivatedcerealcropsworldwide,providingastaplefoodsourceforasignificantportionoftheworld'spopulation.Starchisthedominantcomponentofwheatgrains,contributingtoitsnutritionalandfunctionalproperties.However,excessivestarchconsumptioncanleadtovarioushealthissues,particularlyrelatedtobloodglucoselevels.Therefore,thedevelopmentofwheatvarietieswithincreasedlevelsofresistantstarch,whichisnotreadilydigestedbyhumanenzymes,hasgainedsignificantattention.Inthispaper,wediscussthegeneticcharacteristicsandmolecularmarkersassociatedwithwheatresistantstarch. 1.Introduction 1.1Wheatasastaplefoodcrop 1.2Thesignificanceofresistantstarchinhumannutrition 1.3Importanceofgeneticcharacterizationandmolecularmarkers 2.GeneticCharacteristicsofWheatResistantStarch 2.1Inheritanceofresistantstarch 2.2Roleofcarbohydrate-activeenzymesinstarchcomposition 2.3Regulatorygenesinvolvedinresistantstarchbiosynthesis 3.MolecularMarkersforWheatResistantStarch 3.1DNAmarkersassociatedwithresistantstarchgenes 3.2Genome-wideassociationstudies(GWAS)forresistantstarch 3.3Molecularmarker-assistedbreedingforselectingresistantstarchtraits 4.ScreeningandSelectionofMolecularMarkers 4.1High-throughputgenotypingtechnologies 4.2Identificationandvalidationofmarker-traitassociations 4.3Pyramidingmultipleresistantstarchallelesusingmarkers 5.FuturePerspectivesandChallenges 5.1Biotechnologicalapproachesforenhancingresistantstarch 5.2Theimpactofresistantstarchonwheatprocessingqualities 5.3Consumeracceptanceofresistantstarch-enrichedwheatproducts 6.Conclusion 6.1Relevanceofgeneticcharacterizationandmolecularmarkers 6.2Potentialbenefitsofresistantstarch-enrichedwheatvarieties 6.3Futuredirectionsforresearchandapplication Keywords:wheat,resistantstarch,geneticcharacteristics,molecularmarkers,genotyping,marker-assistedbreeding Introduction: Wheatisaversatilecropwidelyusedintheproductionofnumerousfooditems,suc