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大白菜离体再生中LBD基因的作用和春化相关BrVIN3基因的遗传转化.docx

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大白菜离体再生中LBD基因的作用和春化相关BrVIN3基因的遗传转化 Title:TheRoleofLBDGenesinDeNovoRegenerationofChineseCabbageandtheGeneticTransformationofBrVIN3GeneinVernalization-AssociatedPathways Abstract: Denovoregenerationisanessentialprocessforthepropagationandgenetictransformationofplants.Chinesecabbage,acommonlyconsumedvegetable,exhibitsremarkableregenerativecapacity,makingitanidealmodeltostudythemolecularmechanismsunderlyingplantregeneration.Lateralorganboundariesdomain(LBD)geneshavebeenimplicatedinvariousdevelopmentalprocesses,includingtissuedifferentiationanddenovoregeneration.ThispaperdiscussestheroleofLBDgenesinthedenovoregenerationofChinesecabbageandexaminesthegenetictransformationofBrVIN3geneinvernalization-associatedpathways. Introduction: Plantregenerationisacomplexbiologicalprocessthatinvolvestheactivationofdormantcellpopulations,cellularreprogramming,andtissuedifferentiation.Chinesecabbage(Brassicarapasubsp.pekinensis)iswell-knownforitscapabilitytodenovoregeneratefromisolatedorgans,suchasleavesandcotyledonexplants.Unravelingthemolecularmechanismsunderlyingthisregenerationprocessisofgreatsignificanceforbothfundamentalresearchandagriculturalapplications. RoleofLBDGenesinDeNovoRegeneration: Lateralorganboundariesdomain(LBD)genesareafamilyoftranscriptionfactorsthatplayacrucialroleinplantdevelopment.InChinesecabbage,LBDgenesaretransientlyupregulatedduringtheearlystagesofdenovoregeneration.StudieshaveshownthattheoverexpressionofLBDgenesenhancestheregenerationefficiencyofChinesecabbageexplants,suggestingtheirimportanceinmediatingtissuedifferentiationandcellfatedetermination. LBDgenesinfluencevariousaspectsofregeneration,includingcelldivision,callusinduction,andshootmeristemformation.Furthermore,LBDgeneshavebeenimplicatedinhormonesignalingpathways,suchasauxinandcytokinin,whicharecriticalforplantregeneration.ThesefindingshighlightthemultifacetednatureofLBDgenesinmediatingdenovoregenerationbyregulatinghormonalresponsesandpromotingtissuedifferentiation. GeneticTransformationofBrVIN3Genein