预览加载中，请您耐心等待几秒...

BADH基因转化羊草(Leymuschinensis)的研究.docx

预览
1/4
2/4
3/4
4/4

在线预览结束，喜欢就下载吧，查找使用更方便

下载提示 文本预览

如果您无法下载资料，请参考说明：

1、部分资料下载需要金币，请确保您的账户上有足够的金币

2、已购买过的文档，再次下载不重复扣费

3、资料包下载后请先用软件解压，在使用对应软件打开

BADH基因转化羊草(Leymuschinensis)的研究 Introduction: Leymuschinensis,commonlyknownasChinesewildrye,isanimportantperennialgrassspeciesinnorthernChinathatiswidespreadacrossvastareasofthegrasslandsintheregion.Thisspeciesisanidealmodelforgrasslandecosystemresearchandmanagementbecauseofitscomprehensiveecologicaladaptability.Itisanexcellentforage&fodderplant,andisusedtopreventsoilerosion,aswellasinremediationofsalineandalkalinesoils.However,despiteitssignificanceingrasslandecosystems,thewildL.chinensislacksresistancetoavarietyofbioticandabioticstressessuchaspestinfections,drought,andlowtemperatures,whichlimitsitscultivationandapplication.Therefore,itisnecessarytointroducestress-resistantgenesintothisspeciestoimproveitsstressresistancethroughgeneticengineering. BADHgeneisanimportantstress-resistantgene,anditsoverexpressionhasbeenfoundtoinducestressresistanceinnumerouscrops.TheBADHgeneencodesbetainealdehydedehydrogenase,anenzymethatplaysanessentialroleinthebiosynthesisofbetaine,acompoundthatregulatesosmoticpressureandplaysacriticalroleinstressresistance.TheintroductionoftheBADHgeneintoplantgenomeshasbeenshowntoeffectivelyimprovethestressresistanceofvariouscrops. Inthisstudy,wepresentthesuccessfultransformationoftheBADHgeneintoL.chinensisbywhichthestressresistanceofthewildplantisimproved.OurobjectivewastoinvestigatetheeffectofBADHgeneoverexpressiononL.chinensisstressresistance,andprovideevidencetosupportthegeneticengineeringofL.chinensisformorewidespreaduseinthegrasslandecosystem. MaterialsandMethods: TransformationVectorConstruction: AvectorexpressingtheBADHgenewasconstructedasfollows:First,theBADHgenewasamplifiedbyreversetranscriptionpolymerasechainreaction(RT-PCR)fromthegenomeofbarley(Hordeumvulgare)whichservedasadonorspecies.TheamplifiedBADHgenewasthenplacedunderthecontroloftheconstitutivepromoterCaMV35S.TheresultingconstructwasinsertedintothepCAMBIA1391vector.Thefinalconstructwasthenverifiedthroughsequencing. GeneticTransformation: TheconstructwasthenintroducedintoL.chinensisle