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酵母拮抗菌和外源水杨酸对核果类果实抗病相关基因的诱导表达.docx

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酵母拮抗菌和外源水杨酸对核果类果实抗病相关基因的诱导表达 Abstract Inthisstudy,theinductionofdiseaseresistance-relatedgenes(DRRGs)instonefruitbyyeastantagonisticbacteriaandexogenoussalicylicacidwasinvestigated.TheresultsshowedthatthecombinedtreatmentofyeastantagonisticbacteriaandexogenoussalicylicacidsignificantlyinducedtheexpressionofDRRGsinpeachandplumfruit.TheexpressionofPR1,PR2,andPR5geneswasupregulatedby10-to30-fold.Inaddition,thetreatmentalsoenhancedtheresistanceofthefruittothepathogenBotrytiscinerea.Ourresultssuggestthatthecombinationofyeastantagonisticbacteriaandexogenoussalicylicacidcanbeusedasapromisingstrategytoenhancethediseaseresistanceofstonefruit. Introduction Stonefruits,suchaspeachandplum,arewidelygrownandwidelyconsumedaroundtheworld.Thesefruitsareimportantfortheirnutritionalvalueandeconomicsignificance.However,stonefruitproductionisoftenlimitedbyvariousdiseasescausedbyfungalpathogenssuchasBotrytiscinerea,Moniliniaspp.,andRhizopusspp.Thesediseasescancausesignificantlossesinyieldandquality,whichhavepromptedthedevelopmentofnewstrategiestoenhancethediseaseresistanceofstonefruits. Plantdefensemechanismsagainstfungalpathogensinvolvetheinductionofavarietyofdefense-relatedgenes,includingpathogenesis-relatedgenes(PRgenes),whichencodeproteinsthatareinvolvedintheplant'sdefenseresponse.Thesegenescanbeinducedbyvariousbioticandabioticstresses,suchaspathogeninfection,mechanicaldamage,andexposuretoplanthormonesandsignalingcompounds. Yeastantagonisticbacteriaandexogenoussalicylicacid(SA)havebeenstudiedextensivelyfortheirabilitytoinducetheexpressionofDRRGsandenhancetheresistanceofplantstovariouspathogens.Yeastantagonisticbacteriaareagroupofbeneficialmicrobesthatcanproduceavarietyofsecondarymetabolitesandenzymesthatareinvolvedinplantdefenseresponses.ExogenousSAisawell-knowninducerofplantdefenseresponsesandhasbeenshowntoenhancetheexpressionofDRRGsinmanyplantspecies. Inthisstudy,weinvestigatedtheeffectsofyeastantagonisticbacteriaandexogenousSAontheinductionofDRRGsinstonefruitandtheirresistancetoth