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急性热应激对番鸭和蛋鸭NPY、NPY5R及CCK基因表达的影响.docx

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急性热应激对番鸭和蛋鸭NPY、NPY5R及CCK基因表达的影响 Abstract TheimpactofacuteheatstressontheexpressionofNPY,NPY5R,andCCKgenesinPekingducksandegg-layingduckswasinvestigated.Theresultsshowedthatacuteheatstresshadasignificanteffectontheexpressionofthesegenesinbothduckspecies.TheexpressionofNPYandNPY5Rgeneswassignificantlyup-regulated,whiletheexpressionofCCKgenewasdown-regulatedunderheatstress.Thesefindingsprovidenewinsightsintothemolecularregulatorymechanismsunderlyingtheresponseofduckstoheatstress. Introduction Heatstressisacommonenvironmentalstressfactorthathasaprofoundimpactonthehealth,growth,andproductionperformanceofpoultryanimals.Pekingducksandegg-layingducksaretwoeconomicallyimportantduckspeciesthatarewidelydistributedthroughouttheworld.Heatstresshasbeenreportedtohavenegativeeffectsonduckgrowth,feedintake,eggproduction,andimmunefunction,whichcanleadtoeconomiclossesforfarmers.Thehypothalamic-pituitary-adrenalaxis(HPA)isawell-knownphysiologicalpathwaythatmediatesthestressresponseinpoultry.Inparticular,theneuropeptideY(NPY)systemandcholecystokinin(CCK)systemhavebeenimplicatedinthestressresponseofbirds.NPYandCCKareinvolvedinregulatingfeedingbehaviorandenergymetabolism.Inaddition,NPYanditsreceptors,NPY1RandNPY5R,havebeenshowntobeinvolvedintheregulationofstressandanxietyinpoultry.However,littleisknownabouttheeffectofacuteheatstressontheexpressionofNPY,NPY5R,andCCKgenesinducks. Materialsandmethods Experimentalanimals Atotalof30Pekingducksand30egg-layingducks(8weeksold)wereobtainedfromalocalduckfarmandkeptundernormalconditionsforoneweekpriortotheexperiment. Experimentaldesign Theducksweredividedintotwogroups:controlgroupandheatstressgroup(n=15pergroup).Theducksintheheatstressgroupweresubjectedtoatemperatureof35°Candarelativehumidityof60%for3hours,whilethecontrolgroupwaskeptundernormalconditions. Afterheatexposure,theduckswereeuthanized,andhypothalamictissueswerecollectedforRNAextractionandgeneexpressionanalysis. RNAextractionandcDNAsynthesis TotalRNAwasextractedfromthehypothalamictissuesus