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大白菜抽薹开花时间与SSR和InDel标记的关联分析.docx

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大白菜抽薹开花时间与SSR和InDel标记的关联分析 Title:AssociationAnalysisofFloweringTimeinChineseCabbage(BrassicarapaL.ssp.pekinensis)withSSRandInDelMarkers Abstract: Chinesecabbageisanimportantvegetablecropworldwide,andunderstandingthegeneticfactorsinfluencingfloweringtimeiscrucialforbreedingprogramsaimedatimprovingyieldandquality.Inthisstudy,weinvestigatedtheassociationbetweenfloweringtimeandSSR(SimpleSequenceRepeat)andInDel(Insertion/Deletion)markersinChinesecabbage.WeusedadiversepanelofChinesecabbageaccessionstoidentifygeneticmarkersassociatedwithfloweringtimetraits.TheresultsrevealedsignificantassociationsbetweencertainSSRandInDelmarkersandfloweringtime,providingvaluableinsightsintothegeneticmechanismscontrollingthistraitinChinesecabbage. Introduction: Floweringtimeisacriticaldevelopmentalmilestoneinplantlifecyclesandplaysavitalroleinreproductivesuccess.Itisacomplextraitinfluencedbymultiplegeneticandenvironmentalfactors.Understandingthegeneticbasisoffloweringtimeisfundamentalforcropimprovementstrategies,asitimpactsthesynchronizationofdevelopmentalstages,seedproduction,andadaptationtodifferentenvironments.Chinesecabbage(BrassicarapaL.ssp.pekinensis)isawell-knownleafyvegetablecropwithsignificanteconomicimportance.Theaimofthisstudywastoinvestigatetheassociationbetweenfloweringtimeandgeneticmarkers(SSRandInDel)inChinesecabbage. Methods: 1.PlantMaterial:AdiversepanelofChinesecabbageaccessions,includingearly,intermediate,andlate-floweringgenotypes,wasselectedforthisstudy. 2.Phenotyping:Floweringtimetraits,suchasdaystoboltinganddaystoflower,wereobservedandrecordedundercontrolledconditions. 3.DNAExtractionandMarkerAnalysis:GenomicDNAwasextractedfromfreshleavesusingacommercialkit.SSRandInDelmarkerswereselectedbasedonpreviousstudiesandgenomicinformationofChinesecabbage.PCRamplificationwasperformedformarkeranalysis,andsubsequentfragmentanalysiswasconductedusingcapillaryelectrophoresis. 4.DataAnalysis:Thegenotypicandphenotypicdatawereanalyzedusingstatisticalsoftware.Correlationanalysis,associa