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农杆菌介导调节基因Lc转化菊花的研究的综述报告.docx

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农杆菌介导调节基因Lc转化菊花的研究的综述报告 Introduction Lcisaregulatorygenethatcontrolstheanthocyaninpigmentationinplants.Anthocyaninisaflavonoidpigmentthatcontributestothered,blue,andpurplecolorsofmanyplanttissues.Itisalsoapolyphenoliccompoundthathasmanybeneficialeffectsonhumanhealth,includingantioxidant,anticancer,andanti-inflammatoryproperties.Therefore,themanipulationofanthocyaninproductioninplantshasbothagronomicandmedicinalsignificance.OnewaytoachievethisisbyusingtheAgrobacterium-mediatedgenetransfertechnique,whichhasbeenwidelyusedinplantgeneticengineering. Agrobacterium-mediatedgenetransfer AgrobacteriumisasoilbacteriathatcantransferapieceofDNAcalledT-DNA(transferDNA)toplantcells.TheT-DNAiscontainedinaregionofthebacterialgenomecalledtheTi-plasmid(tumor-inducingplasmid),whichalsocontainsseveralothergenesthatareinvolvedinthetransformationprocess.TheT-DNAisintegratedintothehostplantgenome,whereitcanbeexpressedandproduceanewphenotype.Thisprocessiscalledtransformationandhasbeenusedextensivelytogeneratetransgenicplantswithdesirabletraitssuchasherbicideresistance,insectresistance,andimprovednutritionalcontent. Agrobacterium-mediatedtransformationofchrysanthemum Chrysanthemumisapopularornamentalplantthatiswidelycultivatedallovertheworld.Ithasmanyflowercolorsandshapesthatarehighlyvaluedbygrowersandconsumers.However,thegeneticimprovementofchrysanthemumhasbeenlimitedbyitslongbreedingcycleandthelackofefficienttransformationmethods.Recently,theAgrobacterium-mediatedgenetransfertechniquehasbeenappliedtochrysanthemumwithsomesuccess.Forexample,transgenicchrysanthemumplantswithincreasedresistancetothefungalpathogenBotrytiscinereahavebeenobtainedbyintroducinggenesencodingchitinaseandβ-1,3-glucanaseintotheplantgenome. Agrobacterium-mediatedtransformationofchrysanthemumwithLcgene TheLcgenehasbeenintroducedintochrysanthemumusingAgrobacterium-mediatedtransformationinseveralstudies.Forexample,Zhangandcolleagues(2010)transformedchrysanthemumwiththemaizeLcgeneandobtainedtransgenicplantswithincreasedlev