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XYZ[V\,JournalofAnhuiAgri.Sci.2014,42(4):1178-1180]^E_566]^`a5… ¬©­®-¯°¢©±²‰³´Š‹µ¶„·;4/f¸Œ¹ 1233 :;,²<=,>?@,A&(1.Ôä@Ûܛ:±ÝL>H4¼XZ)*,ÔäÞÙ416000;2.ÞÙ#$=€|²²ÄÔä @W“ìrß,Ôäà*D427000;3.à*Dþ1ó$m#j,Ôäà*D427000) 67[žW]B’CADE-FGøAHIÓ?@ԂJäKLMÖSWwãNØÙ。[©Ó]âOP、QRKLJäSÖ]íT,U KLäMÖâ60%WÌVWøXYÇZE1h,‹ú[’SPE\]^_`aÅ,âC18bAHI](4.6mm×250.0mm,5.0μm),0.2% cPFÌd78eÝfg、hi]1.2ml/min、j:kÔÇl260nm,?@Ԃ43JäwãNØÙ。[IJ]ímþn,KLäMÖS4 3JäwãNWopqÒrGs]Jät96.7%、uvwt97.9%、Jätx102.2%、uvwë103.1%。[I"]y©Óy?@Ԃ KLäMÖS43JäwãN<G,zYñÜ,{€…|。 89:CADE;FGøAHI;KLäMÖ;JäwãN ;<4=>S509.9;O657.7+2?@ABCA?DE>0517-6611(2014)04-01178-03 SimultaneouslyDeterminationforFourKindsofSoybeanIsoflavonesofFermentedSoybeanProductsbySolid-phaseExtraction (SPE)andHighPerformanceLiquidChromatography(HPLC) KUANGYongetal(WulingSunshineBiotechnologyCo.Ltd.,Jishou,Hunan416000) Abstract[Objective]TosimultaneouslydetermineisoflavonescontentoffermentedsoybeanproductsbySPEandHPLC.[Method]Thefer mentedsoybeanproductsareextractedfor1hourby60%ethanolsolution,afterdiluted,carryoutcleaningtreatmentbySPEcolumnandcar ryoutgradientelutionbyC18reversedphasechromatographiccolumn(4.6mm×250.0mm,5.0μm),0.2%formicacidandacetonitrile, withflowvelocityof1.2ml/minandUVdetectionwavelengthof260nm,theaveragerecoveryrateoffourcomponentsisdaidzin96.7%, genistoside97.9%,daidzein102.2%andgenistein103.1%.[Conclusion]Themethodcansimultaneouslydeterminefourkindsofsoybean isoflavonescontent,thedataisaccurateandreproducibilityisgood. KeywordsSolidphaseextraction(SPE);Highperformanceliquidchromatography(HPLC);Fermentedsoybeanproducts;Soybeanisoflavones #´`“á(ISO)T#´ÊKi+W;Ky0E266µÚ;‘$ÍÐÚ;+ê,¾/÷。(6û:#´ã(qÿ V,:;„N6V7ÄÅâã-¬ä`“ãç#´ãvxê≥98%)、å.žã(qÿ≥98%)、#´ãX(qÿ≥ )¬ä`“2(á™å.ž2)ç#´2。j¡_`,EC#98%)、å.ž2(qÿ≥98%),J"78>H²Ä¼kX ´`“áK>ö„NXæ’|、æçè、$‘éê2BE、ëZ)*。:;mn:9:(€Mq)、v;(€Mq)、v: D…ì|、íîÐïµxb、æð¦ñOZxò[1-3]。Q(VNq)、gqB。SPEü$:CLEANUPCEC18156、“) óXôõ-.Z[,#´`“áÊ`“áãXa`“áÄÅSEPAX、500mg/6ml。 âãp>ön¶˜JK[4-5],ý5,`“áãX—XbÆK1.2A¤º¢W»„11/100000ÕEVB<¥™¾# æ’|„Nç8÷køùúKt`NûëÞ(,gh`“á´ã、å.ž