茶叶中茶多糖的提取和测定方法.pdf

432中国卫生检验杂志2004年8月第14卷第4期ChineseJournalofHealthLaboratoryTechnology20041August1141No14

续表2[3]BlairHC,JordanSE,PetersonTG,etal.Variableeffectoftyrosinekinase
大豆异黄酮本低值加标量测定均值回收率%RSD%inhibitorsonavianosteoclasticactivityandreductionofbonelossino2
variectomizedrats[J].JCellBiochem,1996,60:1761.
染料木甙0.050.045.891.94.7
[4]LigginsJ,BluckLJ,CowardWA,etal.Extrctionandquantificationof
100.094.394.35.4
daidzeinandgennisteininfood.AnaliticalBiochemistry[J],1998,264(1):
金雀异黄素
298.0100.039395.01.51.
200.049096.22.2[5]FrankeAA,CusterLJ,WangW,etal.HPLCanalysisofisoflavonoidsand
2.6方法的精密度对试样同时进行6次测定,结果见表3。otherphenolicagentsfromfoosandfromhumanfluids.ProcSocExpBiol
四种异黄酮测定结果的重现性均符合分析要求。Med[J],1998,217(3):263.
表3方法的精密度结果(μg/g)[6]FrankeAA,CusterLJ,CernaCM,etal.RapidHPLCanalysisofdietary
phytoestrogensfromlegumesandfromhumanurine.ProcSocExpBiolMed
异黄酮123456平均值RSD%
[J],1995,208(1):18.
Daidzin77.774.168.676.977.181.576.05.7
[7]BarnesS,CowardL,KirkM,etal.HPLC-Massspectrometryanalysisof
Gennistin45.844.248.946.147.743.046.04.7
isoflavones.ProcSocExpBiolMed[J],1998,217(3):254.
Daidzein78.978.578.179.478.177.778.41.0
[8]NurmiT,MazurW,HeinonnenS,etal.Isoflavonecontentofsoybasedsup2
Genistein49.249.850.548.948.147.749.02.1
plements.JPharmBiomedAnal[J],2002,28(1):1.
2.7实际样品测定采用本方法对6份样品进行测定,检测结[9]AussenacT,LacombeS,andDaydéJ.Quantificationofisoflavonesbycapil2
果表明,由于大豆异黄酮在常温状态下稳定,检测方法重现性laryzoneelectrophoresisinsoybeanseeds:effectsofvarietyandenviron2
好,与标示值相基本吻合。ment.AmJClinNutr[J],1998,68(6suppl):1480.
参考文献[10]孙梅君,骆炼,史长颖,等.中国大豆制品中异黄酮含量测定分析
[1]MessinaM,PerskyV,SetchellKDR,etal.Soyintakeandcancerrisk:Are2研究[J].食品与发酵工业,1999,26(5):14.
viewoftheinvitroandinvivodata[J].NutrCancer,1994,21:113.[11]常凤启,韩会新,陈贵茹,等.HPLC法测定食品中金雀异黄素[J].
[2]AndersonJW,JohnstoneBM,Cook-NewellME.Meta-analysisoftheef2中国食品卫生杂志,2003,15(6):500.
fectsofsoyproteinintakeonserumlipids[J].NewEnglJMed,1995,332:[12]RostagnoMA,PalmaM,BarrosoCG.Ultrasound-assistedextractionof
276.soyisoflavones[J].JChromatogrA,2003,1012(2):119.
(收稿日期:2003